Membrane associated enzyme complexes containing lambda type immunoglobulin A

Clinica Chimica Acta; International Journal of Clinical Chemistry
K Sudo, T Kanno

Abstract

The properties of the membrane associated enzyme complexes were investigated. The enzyme complexes of alkaline phosphatase, arylamidase, and L-gamma-glutamyltranspeptidase were excluded by gel filtration on Sephadex G-200. The electrophoretic mobility of the enzyme complexes was found to be in the alpha-1 portion and these enzyme activities were immunologically fixed by anti-IgA and anti-light chain lambda antibodies. Heterogeneity in density was observed by density gradient ultracentrifugation. The activities of membrane-associated enzymes were demonstrated in both high (d > 1.21) and low density particles (1.063 < d < 1.21), and the enzyme activities of a high density particle were immunologically fixed by anti-IgA and anti-light chain lambda antibodies. The heterogeneity in density was based on the presence of immunoglobulin A molecule in the complexes. The specificity of the immunoglobulin which was found in the enzyme complexes proved to be exclusively of the alpha heavy chain classes and lambda light chain types respectively. This isotype specificity of immunoglobulin A is similar to the specificity observed in enzyme-linked immunoglobulins, and simple non-specific binding of immunoglobulin and membrane moiety was elimina...Continue Reading

References

Apr 1, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·T Kanno, K Sudo
Nov 1, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y KajitaY Ochi
Sep 15, 1979·Clinica Chimica Acta; International Journal of Clinical Chemistry·J BohnerM Eggstein
Mar 15, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·D BrocklehurstS R Aparicio
Mar 24, 1975·Clinica Chimica Acta; International Journal of Clinical Chemistry·M E De BroeR J Wieme
Nov 15, 1975·Clinica Chimica Acta; International Journal of Clinical Chemistry·M Nagamine, S Okuma
Feb 1, 1970·Clinica Chimica Acta; International Journal of Clinical Chemistry·J Biewenga, L G Thijs
Nov 9, 1970·Biochemical and Biophysical Research Communications·P D House, M J Weidemann
Nov 1, 1974·American Journal of Clinical Pathology·D P Rhone, F M White
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Oct 14, 1969·Annals of the New York Academy of Sciences·H SuzukiT Oda
Oct 1, 1964·Journal of Chromatography·C J MORRIS

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Citations

Sep 1, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·N E Huseby
Dec 30, 2014·Biochimica Et Biophysica Acta·Takanori MoriyamaNaoyuki Honma

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