Membrane-associated heparan sulfate is not required for rAAV-2 infection of human respiratory epithelia.

Virology Journal
Michael P BoylePamela L Zeitlin

Abstract

Adeno-associated virus type 2 (AAV-2) attachment and internalization is thought to be mediated by host cell membrane-associated heparan sulfate proteoglycans (HSPG). Lack of HSPG on the apical membrane of respiratory epithelial cells has been identified as a reason for inefficient rAAV-2 infection in pulmonary applications in-vivo. The aim of this investigation was to determine the necessity of cell membrane HSPG for efficient infection by rAAV-2. Rates of transduction with rAAV2-CMV-EGFP3 in several different immortalized airway epithelial cell lines were determined at different multiplicities of infection (MOI) before and after removal of membrane HSPG by heparinase III. Removal of HSPG decreased the efficacy of infection with rAAV2 by only 30-35% at MOI < or = 100 for all of respiratory cell lines tested, and had even less effect at an MOI of 1000. Studies in mutant Chinese Hamster Ovary cell lines known to be completely deficient in surface HSPG also demonstrated only moderate effect of absence of HSPG on rAAV-2 infection efficacy. However, mutant CHO cells lacking all membrane proteoglycans demonstrated dramatic reduction in susceptibility to rAAV-2 infection, suggesting a role of membrane glycosaminoglycans other than HSP...Continue Reading

References

Apr 1, 1991·American Journal of Respiratory Cell and Molecular Biology·P L ZeitlinW B Guggino
Nov 28, 1986·Science·Q J SattentauP C Beverley
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J D EskoW H Taylor
Feb 1, 1993·AIDS Research and Human Retroviruses·M PatelM A Norcross
Mar 31, 1998·The Journal of General Virology·L C ImmergluckB C Herold
Jan 12, 1999·Nature Medicine·C SummerfordR J Samulski
Feb 19, 2000·Gene Therapy·P E Monahan, R J Samulski
Feb 23, 2000·Journal of Virology·J S BartlettR J Samulski
Jun 7, 2000·The Journal of Clinical Investigation·D DuanJ F Engelhardt
Jul 18, 2001·The Journal of Clinical Investigation·J D Esko, U Lindahl
Jul 18, 2001·The Journal of Clinical Investigation·E Forsberg, L Kjellén
Sep 27, 2003·Journal of Virology·A KernJ A Kleinschmidt

❮ Previous
Next ❯

Citations

Jul 7, 2019·European Journal of Human Genetics : EJHG·Theresa Mihalic MosherKim L McBride
Apr 10, 2019·Gene Therapy·Bradley A HamiltonJoseph Zabner

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
transfection

Software Mentioned

STATA
Cell Quest

Related Concepts

Related Feeds

Cell-Type Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Cell-Type-Specific Viral Vectors (ASM)

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Cell-Type-Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.