Membrane association and functional regulation of Sec3 by phospholipids and Cdc42

The Journal of Cell Biology
Xiaoyu ZhangWei Guo

Abstract

The exocyst is an octameric protein complex implicated in tethering post-Golgi secretory vesicles at the plasma membrane in preparation for fusion. However, it is not clear how the exocyst is targeted to and physically associates with specific domains of the plasma membrane and how its functions are regulated at those regions. We demonstrate that the N terminus of the exocyst component Sec3 directly interacts with phosphatidylinositol 4,5-bisphosphate. In addition, we have identified key residues in Sec3 that are critical for its binding to the guanosine triphosphate-bound form of Cdc42. Genetic analyses indicate that the dual interactions of Sec3 with phospholipids and Cdc42 control its function in yeast cells. Disrupting these interactions not only blocks exocytosis and affects exocyst polarization but also leads to defects in cell morphogenesis. We propose that the interactions of Sec3 with phospholipids and Cdc42 play important roles in exocytosis and polarized cell growth.

References

Dec 8, 1995·The Journal of Biological Chemistry·P D BurbeloA Hall
Dec 31, 1997·Genes & Development·J L BrownM Peter
Aug 7, 1999·The Journal of Biological Chemistry·W GuoP Novick
Nov 13, 1999·Nature Cell Biology·S R Pfeffer
May 10, 2000·Trends in Cell Biology·W GuoP Novick
Sep 20, 2000·The Journal of Cell Biology·R RohatgiM W Kirschner
Apr 3, 2001·Nature Cell Biology·W GuoP Novick
Oct 12, 2001·The Journal of Biological Chemistry·X ZhangW Guo
Mar 24, 2004·International Review of Cytology·Shu-Chan HsuWei Guo
May 26, 2004·Methods : a Companion to Methods in Enzymology·P T TranFred Chang
Oct 12, 2004·Annual Review of Cell and Developmental Biology·David PruyneAnthony Bretscher
Jan 14, 2005·Molecular Biology of the Cell·Allison ZajacWei Guo
Mar 25, 2005·The Journal of Biological Chemistry·Xiaoyu ZhangWei Guo
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·David E Levin
Feb 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·David E Hokanson, E Michael Ostap
Jul 11, 2006·Nature Structural & Molecular Biology·Mary Munson, Peter Novick

❮ Previous
Next ❯

Citations

Oct 15, 2011·Protoplasma·Jianglan Liu, Wei Guo
May 24, 2012·Nature Reviews. Molecular Cell Biology·Dara Leto, Alan R Saltiel
Jan 20, 2009·Nature Structural & Molecular Biology·Arati TripathiFrederick M Hughson
Jan 12, 2010·Nature Structural & Molecular Biology·Masami YamashitaShuya Fukai
Jul 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Neil VasanKarin M Reinisch
Feb 9, 2010·The Journal of Biological Chemistry·Kyuwon BaekRoberto Dominguez
Jan 15, 2010·Molecular & Cellular Proteomics : MCP·Thiemo B SchreiberHenrik Daub
Jul 10, 2013·The Journal of Cell Biology·Guangzuo LuoWei Guo
Dec 17, 2008·Molecular Biology of the Cell·Jennifer A Songer, Mary Munson
Nov 6, 2009·Molecular Biology of the Cell·Nicholas J Andersen, Charles Yeaman
Mar 20, 2010·Molecular Biology of the Cell·Lacramioara FabianJulie A Brill
May 14, 2010·Molecular Biology of the Cell·Christopher M Hickey, William Wickner
Dec 15, 2010·Molecular Biology of the Cell·Felipe O Bendezú, Sophie G Martin
Feb 11, 2012·Molecular Biology of the Cell·Miriam EssidThierry Soldati
Feb 11, 2012·Molecular Biology of the Cell·Casey A YdenbergMark D Rose
Jul 7, 2012·Molecular Biology of the Cell·Derek McCuskerDouglas Kellogg
Dec 1, 2012·Molecular Biology of the Cell·Marion Weber-BoyvatJussi Jäntti
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·Kelly Orlando, Wei Guo
Jun 28, 2012·PLoS Computational Biology·William R HolmesLeah Edelstein-Keshet
Jun 16, 2012·Genetics·Erfei Bi, Hay-Oak Park
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Lene N Nejsum, W James Nelson
Feb 7, 2014·Molecular Biology of the Cell·David Caballero-Lima, Peter E Sudbery
Jan 4, 2013·Molecular Biology of the Cell·Matyás FendrychViktor Zársky
Jul 7, 2009·Annual Review of Cell and Developmental Biology·I-Mei Yu, Frederick M Hughson
Oct 15, 2014·The Journal of Cell Biology·Dongmei Liu, Peter Novick
Sep 19, 2014·Molecular Biology of the Cell·Guangzuo LuoWei Guo
Aug 15, 2012·The Journal of Cell Biology·Aurélia VernayRobert A Arkowitz
Feb 13, 2013·The Journal of Cell Biology·Mini JoseDerek McCusker
Dec 17, 2008·The Journal of Cell Biology·Dan BairdScott D Emr
Jul 1, 2015·Proceedings of the National Academy of Sciences of the United States of America·Vikram GhugtyalRobert Alan Arkowitz
Sep 28, 2015·Protoplasma·David G RobinsonLiwen Jiang

❮ Previous
Next ❯

Methods Mentioned

BETA
GTPases
fluorescence recovery after photobleaching
fluorescence microscopy
Fluorescence
electron microscopy
PCR

Software Mentioned

SigmaPlot
Image Quant
OpenLab
Improvision

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.