Membrane association, electrostatic sequestration, and cytotoxicity of Gly-Leu-rich peptide orthologs with differing functions

Biochemistry
Damien VanhoyeP Nicolas

Abstract

The skins of closely related frog species produce Gly-Leu-rich peptide orthologs that have very similar sequences, hydrophobicities, and amphipathicities but differ markedly in their net charge and membrane-damaging properties. Cationic Gly-Leu-rich peptides are hemolytic and very potent against microorganisms. Peptides with no net charge have only hemolytic activity. We have used ancestral protein reconstruction and peptide analogue design to examine the roles of electrostatic and hydrophobic interactions in the biological activity and mode of action of functionally divergent Gly-Leu-rich peptides. The structure and interaction of the peptides with anionic and zwitterionic model membranes were investigated by circular dichroism with 2-dimyristoyl-sn-glycero-3-phosphatidylcholine or 1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol vesicles and surface plasmon resonance with immobilized bilayers. The results, combined with antimicrobial assays, the kinetics of bacterial killing, and membrane permeabilization assays, reveal that Gly, Val, Thr, and Ile can all be accommodated in an amphipathic alpha helix when the helix is in a membrane environment. Binding to anionic and zwitterionic membranes fitted to a 2-stage interaction mod...Continue Reading

References

Feb 1, 1977·Quarterly Reviews of Biophysics·G Ehrenstein, H Lecar
Nov 1, 1984·Biomedical Mass Spectrometry·P Roepstorff, J Fohlman
Jan 26, 1996·The Journal of Biological Chemistry·T A MirzabekovB L Kagan
Jun 17, 1998·The Journal of Biological Chemistry·S CharpentierA Delfour
Oct 17, 1998·Biochimica Et Biophysica Acta·C Wechselberger
Nov 7, 1998·Biochimica Et Biophysica Acta·K Matsuzaki
Jun 6, 2000·American Journal of Physiology. Cell Physiology·J I Kourie, A A Shorthouse
Aug 10, 2000·Biochimica Et Biophysica Acta·K MatsuzakiS Kobayashi
Apr 20, 2001·Seminars in Respiratory Infections·T Ganz
Oct 31, 2001·European Journal of Biochemistry·A GiangasperoA Tossi
Feb 16, 2002·The Journal of Biological Chemistry·Irina KustanovichAmram Mor
Oct 11, 2002·The New England Journal of Medicine·Michael Zasloff
Dec 5, 2002·Current Drug Targets. Infectious Disorders·R E W Hancock, A Patrzykat
Dec 5, 2002·The Journal of Allergy and Clinical Immunology·Richard L GalloMohamed Zaiou
Jul 23, 2003·Protein and Peptide Letters·Anna Christina C NascimentoMariana S Castro
Oct 17, 2003·Protein and Peptide Letters·Sarah R DennisonDavid A Phoenix
Nov 7, 2003·International Journal of Antimicrobial Agents·Kim A BrogdenBrian F Tack

❮ Previous
Next ❯

Citations

Oct 26, 2012·Journal of Chemical Information and Modeling·Nédia KamechDavor Juretić
May 23, 2012·International Journal of Peptides·C PolancoJ A Castanon-Gonzalez
Mar 1, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hiroshi KamimoriMarie-Isabel Aguilar
Jun 16, 2009·Toxicon : Official Journal of the International Society on Toxinology·Yi-Ling ChiouLong-Sen Chang
Mar 21, 2009·Toxicon : Official Journal of the International Society on Toxinology·Juliana C SousaKrishnamurti M Carvalho
Aug 13, 2009·Toxicon : Official Journal of the International Society on Toxinology·Pei-Hsiu KaoLong-Sen Chang
Dec 23, 2008·Toxicon : Official Journal of the International Society on Toxinology·Pei-Hsiu KaoLong-Sen Chang
Oct 28, 2005·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Jul 19, 2008·The FEBS Journal·Constance AuvynetYvonne Rosenstein
Oct 22, 2008·Biochimica Et Biophysica Acta·Pierre Nicolas, Chahrazade El Amri
Feb 9, 2019·Journal of Peptide Science : an Official Publication of the European Peptide Society·Jelena PanticJ Michael Conlon
Nov 7, 2019·International Journal of Molecular Sciences·Peicho PetkovLeandar Litov
Sep 19, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·J P MichelV Rosilio

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.