Membrane association of monotopic phosphoglycosyl transferase underpins function

Nature Chemical Biology
Leah C RayKaren N Allen

Abstract

Polyprenol phosphate phosphoglycosyl transferases (PGTs) catalyze the first membrane-committed step in assembly of essential glycoconjugates. Currently there is no structure-function information to describe how monotopic PGTs coordinate the reaction between membrane-embedded and soluble substrates. We describe the structure and mode of membrane association of PglC, a PGT from Campylobacter concisus. The structure reveals a unique architecture, provides mechanistic insight and identifies ligand-binding determinants for PglC and the monotopic PGT superfamily.

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Citations

May 31, 2020·The Journal of Biological Chemistry·Krista M ArmbrusterTimothy C Meredith
May 20, 2020·The Journal of Biological Chemistry·Chris WhitfieldSteven D Kelly
Jul 19, 2020·Annual Review of Microbiology·Chris WhitfieldCaitlin Sande
Mar 31, 2019·Nature Communications·Marvin RauschTanja Schneider
Apr 6, 2019·Acta Crystallographica. Section F, Structural Biology Communications·Florian BleffertFilip Kovacic
Dec 19, 2020·FEMS Microbiology Reviews·Tong SuLok-To Sham
Jan 22, 2021·Proceedings of the National Academy of Sciences of the United States of America·Katherine H O'TooleKaren N Allen
Dec 14, 2019·Cell Chemical Biology·Jean-Marie SwiecickiBarbara Imperiali
Jun 9, 2021·Biochemical Society Transactions·Katherine H O'TooleBarbara Imperiali

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BETA
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Software Mentioned

RaptorX
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Rosetta
PyMol
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Phenix
SHELXD
UCSF Chimera

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