PMID: 7008848Dec 31, 1980Paper

Membrane asymmetry. A survey and critical appraisal of the methodology. II. Methods for assessing the unequal distribution of lipids

Biochimica Et Biophysica Acta
A H Etemadi

Abstract

In the companion paper, I have reviewed the techniques employed for assessment of the asymmetric distribution and orientation of membrane proteins. This article deals with methods applicable to the investigation of the unequal distribution of lipids between the two membrane leaflets. Among the techniques I will discuss are the use of immunological techniques and lectins, chemical reagents, enzymatic isotopic labeling and degradation of membrane lipids, exchange proteins and physical techniques. Whenever appropriate, problems of crypticity and non-availability of lipids to interact with the appropriate ligands, reagents, modifying enzymes or exchange proteins have been envisaged. It appears that in many case, highly discordant results, sometimes with the same biological material, have been obtained. Some of the difficulties encountered presumably stem from the reported existence of non-bilayer arrangements and isotropic movement of lipids as evidenced by freeze-fracture and NMR studies. Other problems may be related to the induction of such arrangements, especially the inverted micellar arrangement, by the modifying agents, particularly degradation enzymes or exchange proteins when they cause severe unilateral modification of th...Continue Reading

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Feb 12, 1990·FEBS Letters·D JeckelF Wieland
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