Membrane-based on-line optical analysis system for rapid detection of bacteria and spores

Biosensors & Bioelectronics
Pierre N FlorianoJohn T McDevitt

Abstract

We report here the adaptation of our electronic microchip technology towards the development of a new method for detecting and enumerating bacterial cells and spores. This new approach is based on the immuno-localization of bacterial spores captured on a membrane filter microchip placed within a flow cell. A combination of microfluidic, optical, and software components enables the integration of staining of the bacterial species with fully automated assays. The quantitation of the analyte signal is achieved through the measurement of a collective response or alternatively through the identification and counting of individual spores and particles. This new instrument displays outstanding analytical characteristics, and presents a limit of detection of approximately 500 spores when tested with Bacillus globigii (Bg), a commonly used simulant for Bacillus anthracis (Ba), with a total analysis time of only 5 min. Additionally, the system performed well when tested with real postal dust samples spiked with Bg in the presence of other common contaminants. This new approach is highly customizable towards a large number of relevant toxic chemicals, environmental factors, and analytes of relevance to clinical chemistry applications.

References

Mar 27, 1997·Journal of Biochemical and Biophysical Methods·K DillJ D Olson
Jun 9, 1998·Journal of Immunological Methods·Jeffrey D Brewster, R S Mazenko
May 8, 1999·Science·Phillip BelgraderFred P Milanovich
Feb 13, 2001·Biomolecular Engineering·M Szczesna-Antczak, E Galas
Feb 24, 2001·Biosensors & Bioelectronics·S S IqbalJ P Chambers
Mar 7, 2001·Applied and Environmental Microbiology·B C WeimerX Wang
Sep 14, 2001·Letters in Applied Microbiology·Sou-ichi MakinoK Takeshi
Sep 15, 2001·Journal of Applied Microbiology·H I CheunK Takeshi
Mar 5, 2002·Letters in Applied Microbiology·J L RadosevichGary L Andersen
Apr 18, 2002·Biosensors & Bioelectronics·Joon Mo YangMichael Krihak
Jun 19, 2002·FEMS Microbiology Reviews·Michael SteinertJörg Hacker
Jul 27, 2002·Analytical Chemistry·Nick ChristodoulidesJohn T McDevitt
Oct 25, 2002·Emerging Infectious Diseases·Peter M DullDavid Ashford

Citations

Oct 31, 2008·Analytical and Bioanalytical Chemistry·Hai-Fang Li, Jin-Ming Lin
Feb 5, 2010·Analytical Chemistry·Jesse V JokerstJohn T McDevitt
Dec 8, 2011·IEEE Pulse·Elain FuJohn T McDevitt
Aug 4, 2009·Applied and Environmental Microbiology·Amanda B HerzogSyed A Hashsham
Mar 25, 2010·Cancer Prevention Research·Shannon E WeigumJohn T McDevitt
Jul 15, 2005·PLoS Medicine·William R RodriguezJohn T McDevitt
Apr 14, 2010·Biomarkers in Medicine·Craig S MillerJohn T McDevitt
Dec 23, 2009·Nanomedicine·Jesse V Jokerst, John T McDevitt
Jan 1, 2009·Sensors·Jürgen MairhoferPeter Ertl
Apr 20, 2010·Journal of Microbiological Methods·Shilpakala Sainath RaoChintamani D Atreya
Jun 16, 2010·Journal of Bioscience and Bioengineering·Yutaka YawataNobuhiko Nomura
Jun 15, 2010·Journal of Bioscience and Bioengineering·Yutaka YawataNobuhiko Nomura
Feb 21, 2007·Microbiology and Immunology·Yoshihito FujinamiJiro Yasuda
Sep 20, 2005·Biosensors & Bioelectronics·Gossett A Campbell, Raj Mutharasan
Aug 18, 2016·Oral Oncology·Timothy J AbramJohn T McDevitt
Nov 1, 2016·Biomedical Microdevices·Xueye ChenXuyao Huo
Jan 12, 2008·Journal of Chromatography. a·Ning BaoChang Lu
Aug 25, 2006·Lab on a Chip·J de JongM Wessling
Jul 27, 2007·Lab on a Chip·Shannon E WeigumJohn T McDevitt
Jan 19, 2008·Electrophoresis·Kimberly D ChichesterChrista L Colyer
Apr 19, 2019·Micromachines·Nicolaos ChristodoulidesJohn T McDevitt

Related Concepts

Cell Density
Real-Time Systems
Medical Device Design
Immunoassay Method
Membranes, Artificial
Fluorescence Spectroscopy
Reproduction Spores
Ultrafiltration
Biosensing Techniques
Prosthesis Failure Analysis

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