PMID: 10161Sep 15, 1976

Membrane-bound DD-carboxypeptidase and transpeptidase activities from Bacillus megaterium KM at pH 7. General properties, substrate specificity and inhibition by beta-lactam antibiotics

European Journal of Biochemistry
A MarquetT Diaz-Mauriño

Abstract

1. The membranes from Bacillus megaterium KM contained a DD-carboxypeptidase with optimum activity under the following conditions: pH 7; ionic strength, 1.3 M; temperature, 40 degrees C and below 20 degrees C. It did not require any divalent cation, but was inactivated by Cu2+ and Hg2+. It was stimulated by 2-mercaptoethanol and low concentrations of p-chloromercuribenzoate. 2. The membrane preparation also catalyzed a simple transpeptidation reaction using as carboxyl acceptors D-alanine or glycine. 3. The conditions for optimum activity, temperature-inactivation, temperature-dependence of the activity, carboxyl donor specificity, sensitivity to beta-lactam antibiotics, and insensitivity to potential peptide inhibitors of both enzyme activities, was identical. The DD-carboxypeptidase showed inhibition by D-alanine and Ac2-L-Lys-D-Ala. 4. The inhibition by beta-lactam antibiotic was reversible for both enzymic activities and the time-dependence for their recovery was identical. 5. The DD-carboxypeptidase was very sensitive to changes in the configuration and size of the side-chains of the C-terminal dipeptide of the substrate. Amino acid residues at the C-terminus that precluded the peptide from being a DD-carboxypeptidase subs...Continue Reading

References

May 10, 1974·Annals of the New York Academy of Sciences·P E Reynolds, H J Barnett
Dec 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·P M Blumberg, J L Strominger
Jan 1, 1973·The Biochemical Journal·M NietoJ M Ghuysen
Jun 1, 1974·The Biochemical Journal·R Eisenthal, A Cornish-Bowden
Sep 2, 1969·Biochimica Et Biophysica Acta·M MatsuhashiB Maruo
Jul 21, 1970·Biochemistry·J Coyette, J M Ghuysen
May 25, 1971·Biochemistry·M Leyh-BouilleM Nieto
Mar 28, 1972·Biochemistry·M Leyh-BouilleH R Perkins
Mar 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·K IzakiJ L Strominger
May 25, 1966·Biochemical and Biophysical Research Communications·Y ArakiE Ito
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May 1, 1962·The Biochemical Journal·R P NOVICK

Citations

Jan 1, 1981·Pharmacology & Therapeutics·F C Neuhaus, W P Hammes
Jan 1, 1985·Pharmacology & Therapeutics·D J Tipper
Oct 1, 1981·European Journal of Biochemistry·A W WykeN A Curtis
Aug 1, 1982·European Journal of Biochemistry·A Rodríguez-TébarD Vázquez
Nov 15, 1983·European Journal of Biochemistry·J A ToddD J Ellar
Apr 15, 1977·FEBS Letters·H A ChaseP E Reynolds
Jan 1, 1985·Critical Reviews in Microbiology·J M Frère, B Joris
Jan 1, 1983·CRC Critical Reviews in Biochemistry·V S Rao, T K Vasudevan
May 7, 2019·MedChemComm·Geir Kildahl-AndersenOve Alexander Høgmoen Åstrand

Related Concepts

Acyltransferase
Bacillus megaterium
Carboxypeptidase
Plasma Membrane
Chloromercuribenzoates
Hydrogen-Ion Concentration
Cell Surface Proteins
2-Mercaptoethanol
Osmolality
Penicillin

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