PMID: 9642199Jun 27, 1998Paper

Membrane-bound lytic endotransglycosylase in Escherichia coli

Journal of Bacteriology
A R KraftJ V Höltje

Abstract

The gene for a novel endotype membrane-bound lytic transglycosylase, emtA, was mapped at 26.7 min of the E. coli chromosome. EmtA is a lipoprotein with an apparent molecular mass of 22kDa. Overexpression of the emtA gene did not result in bacteriolysis in vivo, but the enzyme was shown to hydrolyze glycan strands isolated from murein by amidase treatment. The formation of tetra- and hexasaccharides, but no disaccharides, reflects the endospecificity of the enzyme. The products are characterized by the presence of 1,6-anhydromuramic acid, indicating a lytic transglycosylase reaction mechanism. EmtA may function as a formatting enzyme that trims the nascent murein strands produced by the murein synthesis machinery into proper sizes, or it may be involved in the formation of tightly controlled minor holes in the murein sacculus to facilitate the export of bulky compounds across the murein barrier.

References

Dec 1, 1975·Journal of Bacteriology·J V HöltjeU Schwarz
Jan 1, 1977·European Journal of Biochemistry·B G Spratt
Jul 25, 1991·Nucleic Acids Research·R H DonJ S Mattick
Sep 1, 1991·Journal of Bacteriology·B Walderich, J V Höltje
Oct 1, 1988·Molecular & General Genetics : MGG·C PotvinG Bellemare
May 14, 1969·Journal of Molecular Biology·U SchwarzH Frank
Apr 1, 1980·Journal of Molecular Biology·M J Casadaban, S N Cohen
May 15, 1981·European Journal of Biochemistry·E H BeacheyU Schwarz
May 1, 1995·Molecular Microbiology·K EhlertM F Templin
Dec 15, 1993·FEMS Microbiology Letters·R E Bishop, J H Weiner
Aug 1, 1993·FEMS Microbiology Letters·T RomeisJ V Höltje
Sep 15, 1995·Structure·G Davies, B Henrissat
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·A R MushegianE W Nester
Oct 1, 1996·Journal of Bacteriology·A J Dijkstra, W Keck
Apr 1, 1996·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·A J Dijkstra, W Keck
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Sep 5, 1997·Science·F R BlattnerY Shao
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·J V Höltje

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Citations

May 16, 2007·The Journal of Biological Chemistry·Karin E van StraatenAndy-Mark W H Thunnissen
Feb 13, 2008·FEMS Microbiology Reviews·Waldemar VollmerSimon Foster
Dec 14, 2001·Journal of Bacteriology·Pia S Rydman, Dennis H Bamford
May 7, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Pramod K Madoori, Andy Mark W H Thunnissen
Nov 21, 2012·Annals of the New York Academy of Sciences·Jarrod W JohnsonShahriar Mobashery
Jul 1, 2014·The FEBS Journal·Massimo SammitoIsabel Usón
Oct 29, 2015·Molecular Microbiology·Rachel YunckThomas G Bernhardt
Oct 6, 2000·Annual Review of Microbiology·M Riley, M H Serres
Aug 10, 2000·Molecular Microbiology·P S Rydman, D H Bamford
Aug 3, 2002·European Journal of Biochemistry·Gary T JenningsGuido Grandi
May 6, 2004·Journal of Bacteriology·Stephen J BrokxJoel H Weiner
Feb 1, 2008·The Journal of Biological Chemistry·Edie M Scheurwater, Anthony J Clarke
Dec 1, 2011·Microbiology and Molecular Biology Reviews : MMBR·Jean van Heijenoort
Dec 6, 2020·Molecular Microbiology·Jessica L BohrhunterThomas G Bernhardt
Jun 24, 2017·Critical Reviews in Biochemistry and Molecular Biology·David A DikShahriar Mobashery
Jul 10, 2019·Molecular Microbiology·Anna I WeaverTobias Dörr
Aug 31, 2000·Analytical Biochemistry·N T Blackburn, A J Clarke
Sep 6, 2005·Journal of Molecular Biology·Karin E van StraatenAndy-Mark W H Thunnissen
Feb 21, 2013·Journal of the American Chemical Society·Mijoon LeeShahriar Mobashery

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