PMID: 6974566Jul 7, 1981Paper

Membrane charge as effector of cytochrome P-450LM2 catalyzed reactions in reconstituted liposomes

Biochemistry
M Ingelman-SundbergJ Rydström

Abstract

The phospholipid specificity of rabbit liver microsomal cytochrome P-450LM2 catalyzed hydroxylation reactions was examined in reconstituted phospholipid vesicles. An apparent linear relationship between the negative charge of the vesicles and the rate of P-450LM2-catalyzed O-dealkylation of p-nitroanisole or 7-ethoxycoumarin was obtained. The membrane charge-mediated increase in hydroxylation activities was found not to be due to (i) an altered lipid/water partition coefficient of the substrate, (ii) a change in the apparent Michaelis constant of P-450LM2 for the substrate, (iii) a different activation energy of the O-demethylation of p-nitroanisole, (iv) different spin states of P-450LM2 or (v) an altered secondary structure of this enzyme as monitored by circular dichroism. However, when the formation of the ferrous carbonyl complex of P-450LM2 was followed under aerobic or anaerobic conditions after the addition of NADPH to the vesicles, an increased negative charge of the membrane was accompanied by an increased reducibility of P-450LM2. A similar linear relationship between the reducibility of cytochrome b5 and the negative charge of the liposomes was also evident in membranes containing NADPH-cytochrome P-450 reductase an...Continue Reading

References

Jan 5, 1979·Biochimica Et Biophysica Acta·C C Cunningham, G Sinthusek
Sep 15, 1975·FEBS Letters·G EytanE Racker
Jan 1, 1977·Journal of Supramolecular Structure·E Racker
Apr 6, 1978·Biochimica Et Biophysica Acta·J W DePierre, L Ernster
Jan 1, 1978·Methods in Enzymology·R A ProughR T Mayer
Jan 1, 1975·Annual Review of Biochemistry·I C GunsalusS G Sligar
Jan 1, 1977·FEBS Letters·M Ingelman-Sundberg, H Glaumann
Nov 28, 1980·Biochemical and Biophysical Research Communications·M Ingelman-Sundberg, I Johansson
Jan 1, 1980·Annual Review of Biochemistry·R E White, M J Coon
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

❮ Previous
Next ❯

Citations

Nov 4, 1986·Biochimica Et Biophysica Acta·A Aström, J W DePierre
May 3, 2003·Pharmacology & Therapeutics·Wayne L Backes, Rusty W Kelley
Jul 15, 1983·European Journal of Biochemistry·M Ingelman-SundbergK Ruckpaul
Nov 15, 1983·European Journal of Biochemistry·W T Forsee, J S Schutzbach
Dec 1, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·James R ReedWayne L Backes
Sep 15, 2001·Archives of Biochemistry and Biophysics·A E AitkenB S Masters
May 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Timothy H Bayburt, Stephen G Sligar
Dec 15, 1993·European Journal of Biochemistry·W G BalversI M Rietjens
Apr 17, 2013·Biotechnology and Applied Biochemistry·Tomas LaursenBirger Lindberg Møller
Aug 15, 1998·Archives of Biochemistry and Biophysics·C H YunF P Guengerich
Dec 2, 2014·Trends in Plant Science·Tomas LaursenJean-Etienne Bassard
May 31, 1982·Biochemical and Biophysical Research Communications·M Ingelman-Sundberg, G Ekström
Nov 4, 2016·The Journal of Physical Chemistry. B·Veronika NavrátilováMichal Otyepka
Sep 3, 2004·Journal of Lipid Research·Dilyara A MurtazinaIrina A Pikuleva
Jan 1, 1993·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·M EngelkeH A Diehl
Oct 27, 2016·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Lauren M Brignac-HuberWayne L Backes
Aug 22, 2013·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Lauren M Brignac-HuberWayne L Backes
Aug 8, 1997·The Journal of Biological Chemistry·C H YunH Kim
Jun 15, 2020·Pharmacology & Therapeutics·Aditi DasHannah C Huff

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.