Membrane charge movement in contracting and non-contracting skeletal muscle fibres

The Journal of Physiology
P Horowicz, M F Schneider

Abstract

1. The single gap voltage clamp technique (Kovács & Schneider, 1978) was used to monitor membrane charge movement in tendon-terminated short segments of cut frog skeletal muscle fibres.2. Experiments were performed both on fibres able to contract and on fibres in which contraction was eliminated by exposing the open end to a solution containing 20 mm-EGTA. In both cases ionic conductances were minimized by using a predominantly caesium glutamate solution at the open end and a predominantly tetraethyl-ammonium sulphate solution with tetrodotoxin at the closed end.3. Modifications of previously used charge movement analysis procedures included synthesis of a ;mean linear' ON and OFF capacitative transient from the OFFs of several different hyperpolarizing pulses and use of only the first 35 msec of the ;mean linear' transient so that base lines could be fitted to unaltered latter parts of ON and OFF currents for depolarizing pulses.4. Simultaneous two-micro-electrode and gap current recording from gap-clamped fibres with blocked contraction established the validity of gap-recorded charge movement currents.5. For pulses to below about 0 mV in non-contracting fibres the charges Q(ON) and Q(OFF) moved by the non-linear transient cur...Continue Reading

Citations

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