Membrane Charging and Swelling upon Calcium Adsorption as Revealed by Phospholipid Nanodiscs

The Journal of Physical Chemistry Letters
Orion ShihOrion Shih

Abstract

Direct binding of calcium ions (Ca2+) to phospholipid membranes is an unclarified yet critical signaling pathway in diverse Ca2+-regulated cellular phenomena. Here, high-pressure-liquid-chromatography, small-angle X-ray scattering (SAXS), UV-vis absorption, and differential refractive index detections are integrated to probe Ca2+-binding to the zwitterionic lipid membranes in nanodiscs. The responses of the membranes upon Ca2+-binding, in composition and conformation, are quantified through integrated data analysis. The results indicate that Ca2+ binds specifically into the phospholipid headgroup zone, resulting in membrane charging and membrane swelling, with a saturated Ca2+-lipid binding ratio of 1:8. A Ca2+-binding isotherm to the nanodisc is further established and yields an unexpectedly high binding constant K = 4260 M-1 and a leaflet potential of ca. 100 mV based on a modified Gouy-Chapman model. The calcium-lipid binding ratio, however, drops to 40% when the nanodisc undergoes a gel-to-fluid phase transition, leading to an effective charge capacity of a few μF/cm2.

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Citations

Aug 14, 2020·The Journal of Physical Chemistry. B·Abhilash Sahoo, Silvina Matysiak
Nov 17, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Evelyne DeplazesAlvaro Garcia

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