Membrane chloride conductance and capacitance in Jurkat T lymphocytes during osmotic swelling

Biophysical Journal
P E RossM D Cahalan


Video microscopy and whole-cell patch-clamp recording were used to monitor changes in relative cell volume (V/Vo), chloride conductance (gCl), and membrane capacitance (Cm) during osmotically induced swelling in Jurkat T lymphocytes. Cellular swelling was initiated with hyperosmotic pipette solutions. Simultaneous evaluation of V/Vo and gCl revealed a 59-s delay between the inception of swelling and the activation of outwardly rectifying, ATP-dependent Cl- channels. Following the delay, increases in V/Vo and gCl progressed in parallel. In contrast, Cm, a measure of cell surface area, fell gradually at a rate of approximately 150 fF/min after whole-cell access was achieved. The decline in Cm lasted 200 s and was followed by a rapid rise (approximately 750 fF/min). The rise in Cm coincided with a variable increase in "leak" current, gCl increased at a slower rate and reached lower peak values in experiments performed without ATP; ATP had no effect on the biphasic Cm time course. The temporal separation of conductance and capacitance during swelling suggests that gCl and Cm vary independently, supporting the hypothesis that a large portion, if not all, of the whole-cell Cl- conductance activated during swelling is provided by volu...Continue Reading


Mar 1, 1996·Pflügers Archiv : European journal of physiology·G L BuschF Lang
Nov 3, 2004·The Journal of Membrane Biology·R ReussV L Sukhorukov
Jun 29, 2007·The Journal of Membrane Biology·Nicolas GroulxRyszard Grygorczyk
Jun 1, 1996·Pflügers Archiv : European journal of physiology·A H HainsworthM A Barrand
Aug 1, 1994·Current Opinion in Cell Biology·T J Jentsch
Jan 1, 1997·Progress in Biophysics and Molecular Biology·B NiliusG Droogmans
Nov 6, 1998·Pharmacology & Therapeutics·A R Wright, S A Rees
Oct 1, 1996·General Pharmacology·B NiliusG Droogmans
Jan 13, 1998·Current Opinion in Biotechnology·M D Cahalan, K G Chandy
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·T VoetsB Nilius
Jul 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·H WulffK G Chandy
Oct 31, 2007·The Journal of General Physiology·Li-Ting Chien, H Criss Hartzell
Nov 27, 2004·The Journal of Physiology·Rodolphe Fischmeister, H Criss Hartzell
Sep 17, 2009·Immunological Reviews·Michael D Cahalan, K George Chandy
Nov 5, 2016·Molecular Biology of the Cell·Lionel GuillouJulien Husson
May 16, 1998·The Journal of Cell Biology·A Lepple-WienhuesF Lang
Oct 13, 2006·Journal of Neurochemistry·Tina PangrsicMarko Kreft
Jan 1, 1994·Protoplasma·B G Pickard
Sep 16, 2003·Nature Neuroscience·Zizhen Zhang, Charles W Bourque
Nov 24, 2006·The Journal of Biological Chemistry·Adrien RenaudoOlivier Soriani
Mar 15, 2003·American Journal of Physiology. Cell Physiology·Margot BräuerHeike Jäger
Apr 9, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Lepple-WienhuesF Lang
Mar 29, 2001·Physiological Reviews·O P Hamill, B Martinac
Jan 22, 1998·The American Journal of Physiology·K J RennieM J Correia
Oct 12, 1999·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·J Markham, J A Conchello
Nov 1, 1996·Glia·C L Bowman, J W Lohr
Nov 14, 1998·The American Journal of Physiology·J DentonW M Moran
Oct 1, 1995·The American Journal of Physiology·J ArreolaP A Knauf

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