PMID: 7523601Nov 1, 1994Paper

Membrane clustering and bungarotoxin binding by the nicotinic acetylcholine receptor: role of the beta subunit

Journal of Neurochemistry
S V WheelerR Foreman

Abstract

Nicotinic acetylcholine receptors (nAChRs) are localised at morphologically distinct regions of the post-synaptic membrane by interactions between the receptor subunits and cytoskeletal proteins, such as the 43-kDa protein. We have used Xenopus oocytes to examine the localisation and pharmacological properties of muscle nAChRs associated with 43-kDa protein and to compare them with hybrid muscle nAChRs containing a beta subunit derived from a neuronal source. Receptors expressed on the oocyte outer membrane were visualised using confocal scanning laser microscopy. Coexpression of mouse muscle subunit alpha 1 beta 1 gamma delta and 43-kDa protein transcripts produced discrete receptor aggregates with a diameter of 1-5 microns whose function was partially blocked by application of neuronal bungarotoxin (NBT) at 100 nM. Substitution of the beta 1 subunit by the neuronal beta 2 protein produced a functioning receptor that did not aggregate in the presence of 43-kDa protein and was substantially blocked by the same concentration of NBT. Hybrid alpha 1 beta 4 gamma delta receptors exhibited a combination of characteristics in that they clustered like normal muscle subunits in the presence of 43-kDa protein, but showed a sensitivity t...Continue Reading

Citations

Feb 6, 2017·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Atqiya AishahRita Machaalani
Jul 27, 2007·The FEBS Journal·Dimitra KalamidaSocrates J Tzartos
Jan 1, 1997·Molecular and Cellular Neurosciences·S H YangW D Phillips
Oct 27, 1998·European Journal of Pharmacology·C M GarlandR J Walker
Jul 9, 1999·Neurochemistry International·V EbertW Sieghart

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