Membrane conductance oscillations induced by serum in quiescent human skin fibroblasts.

The Journal of Physiology
T Pallotta, A Peres

Abstract

1. Application of fetal calf serum to quiescent human fibroblasts, kept under whole-cell voltage clamp at positive potentials, induced a series of transient rises in membrane conductance. 2. The first transient increase in conductance developed with very short time lag (2-10 s) after serum addition, while the period between successive transients was 30-90 s, being remarkably constant in each particular cell. 3. Raising the Ca2(+)-buffering capacity of the intracellular solution with 1 mM-EGTA suppressed the appearance of the sustained oscillations. 4. The conductance increase was strongly voltage dependent: voltage ramps applied before, during and after the transients revealed the activation of an outwardly rectifying conductance with variable reversal potentials (between +14 and -55 mV). 5. No significant shifts of the reversal potential were observed when the extracellular K+ concentration was increased to 126 mM. Substitution of K+ with Cs+ as intracellular cation eliminated the outward current in response to serum. 6. External application of the Ca2+ ionophore A23187 elicited currents which were very similar in voltage dependence and time course to those triggered by serum. 7. The serum-induced response persisted unaffected...Continue Reading

Citations

Jan 1, 1993·Progress in Biophysics and Molecular Biology·J M Dubois, B Rouzaire-Dubois
May 28, 2013·Cellular Signalling·Rama K MallampalliMarianna Agassandian
Dec 9, 1992·Biochimica Et Biophysica Acta·D LovisoloG Bonelli
Feb 17, 1992·Biochimica Et Biophysica Acta·D LovisoloL Munaron
Oct 1, 1992·Experimental Cell Research·S GiovannardiA Peres

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