PMID: 6162961Dec 30, 1980Paper

Membrane current and noise measurements in voltage-clamped node of Ranvier

The Journal of Membrane Biology
R J van den Berg, W H Rijnsburger

Abstract

In voltage-clamp configurations for nodes of Ranvier the axoplasm resistance functions as a voltage-current converter. In existing configurations this resistance cannot be measured directly. In the present arrangement the electrical resistances of the preparation (axoplasm, membrane and seals) can be measured only from two measurements. This allows us to: 1. calibrate the ionic current under voltage-clamp conditions, and 2. calculate the intensity of the current fluctuations, not arising from the membrane (background noise). The measured axoplasm resistances are considerably higher than the values calculated on the basis of fiber geometry and axoplasm resistivity. The difference is due to the presence of constrictions in the nerve fiber. Membrane current estimation based on geometrical parameters in the presence of wide seals may contain large errors. Variations in the axoplasm resistance for voltage-membrane current conversion were observed within 1.5 hr. In 68% of the fibers this resistance decreased with 30% of the original value. With our current calibration the values for the maximum sodium conductance gNa (at 0mV membrane potential), maximum potassium conductance gK and leakage conductance gL are 49.5 X 10(-8), 6.66 X 10(...Continue Reading

References

Jun 10, 1975·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·W NonnerR Stämpfli
Jul 1, 1979·The Journal of Physiology·S Y ChiuD Stagg
Nov 16, 1979·Biochimica Et Biophysica Acta·B NeumckeR Stämpfli
Jan 1, 1975·Pflügers Archiv : European journal of physiology·W NonnerH Stämpfli
Mar 30, 1973·Annals of the New York Academy of Sciences·D O CarpenterA F Bak
Dec 1, 1971·The Journal of General Physiology·B Hille
Jan 1, 1969·Pflügers Archiv : European journal of physiology·W Nonner
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Aug 29, 1958·The Journal of Physiology·F A DODGE, B FRANKENHAEUSER
Sep 1, 1963·Kybernetik· TEN HOOPENH A REUVER

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Citations

Aug 2, 1990·European Journal of Pharmacology·M G Van ErpR J Van den Berg
Mar 1, 1982·Acta Physiologica Scandinavica·R G SommerE Koppenhoefer
Jan 1, 1982·The Journal of Membrane Biology·G de Bruin

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