Membrane currents and morphological properties of neurons and glial cells in the spinal cord and filum terminale of the frog

Neuroscience Research
A ChvátalE Syková

Abstract

Using the patch-clamp technique in the whole-cell configuration combined with intracellular dialysis of the fluorescent dye Lucifer yellow (LY), the membrane properties of cells in slices of the lumbar portion of the frog spinal cord (n=64) and the filum terminale (FT, n=48) have been characterized and correlated with their morphology. Four types of cells were found in lumbar spinal cord and FT with membrane and morphological properties similar to those of cells that were previously identified in the rat spinal cord (Chvátal, A., Pastor, A., Mauch, M., Syková, E., Kettenmann, H., 1995. Distinct populations of identified glial cells in the developing rat spinal cord: Ion channel properties and cell morphology. Eur. J. Neurosci. 7, 129-142). Neurons, in response to a series of symmetrical voltage steps, displayed large repetitive voltage-dependent Na(+) inward currents and K(+) delayed rectifying outward currents. Three distinct types of non-neuronal cells were found. First, cells that exhibited passive symmetrical non-decaying currents were identified as astrocytes. These cells immunostained for GFAP and typically had at least one thick process and a number of fine processes. Second, cells with the characteristic properties of r...Continue Reading

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Citations

Jun 14, 2005·Hearing Research·Thomas Templin, Andrea Megela Simmons
Jul 26, 2008·Stem Cells and Development·Mercy VargheseIver A Langmoen
Jan 3, 2012·Journal of Neurosurgery. Pediatrics·Samson Sujit Kumar GaddamVedantam Rajshekhar
Oct 13, 2004·Journal of Neuroscience Research·Alexandr ChvátalEva Syková
Nov 13, 2014·Journal of Morphology·Masato UeharaHarumi Sakai
Apr 15, 2008·Brain Research·Csaba BorosMiklós Réthelyi
Aug 12, 2016·The Journal of Comparative Neurology·Ryan ChrenekDavid L Cardozo
Jun 2, 2006·International Review of Neurobiology·Rüdiger SchweigreiterRobert M Gould
Nov 19, 2017·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Jami Bahney, Christopher S von Bartheld
Apr 19, 2007·British Journal of Neurosurgery·G Samson Sujit KumarK Srinivasa Babu

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