Membrane currents elicited by divalent cations in Xenopus oocytes.

The Journal of Physiology
R MilediR M Woodward

Abstract

1. Membrane currents were recorded from voltage-clamped Xenopus oocytes in response to bath application of various divalent cations. 2. In oocytes from 93 of 160 frogs tested, Co2+ ions evoked slow, oscillatory membrane currents. Sensitivity to Co2+ varied greatly between oocytes from different frogs, but was relatively consistent for oocytes taken from the same ovary. Oocytes with high sensitivity had response thresholds of 5-10 microM, and gave currents greater than 1 microA to 1 mM-CoCl2. In contrast, oocytes from some frogs gave no oscillatory response even to 10 mM-CoCl2. With responsive oocytes, Cd2+, Ni2+, Zn2+, Mn2+ and Cr2+ ions (5 microM to 1 mM) also elicited oscillations, whereas Sr2+, Ba2+ and Ca2+ (0.1-10 mM) showed very little activity, and Mg2+ ions, none. 3. Responses to divalent cation were well preserved in defolliculated oocytes, indicating they were generated in the oocyte membrane itself, and were not dependent on the presence of enveloping follicular cells. 4. The oscillatory currents reversed around -20 mV (the chloride equilibrium potential) and rectified strongly at potentials more negative than about -60 mV. The oscillations were mimicked by intraoocyte injection of inositol 1,4,5-trisphosphate (IP3),...Continue Reading

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