Membrane-cytoskeleton interactions: inhibition of odontoblast differentiation by a monoclonal antibody directed against a membrane protein

Differentiation; Research in Biological Diversity
H LesotJ V Ruch

Abstract

It is known that high-molecular-weight (HMW) membrane proteins mediate interactions with constituents of the extracellular matrix and/or with cytoskeletal elements. To study participation of HMW membrane proteins in odontoblast or ameloblast differentiation, an immunological approach has been adopted. Antibodies directed against membrane proteins (Mr, 110-190) from mouse embryos have been produced by the hybridoma technique. Supernatants of hybridoma cultures were screened for their ability to stain dental tissues and also tested for their biological activities on dental cells in primary culture or on developing tooth germs in organ culture. An IgM monoclonal antibody, MC16A16, directed against a 165-kDa antigen present in plasma membrane preparations, reacted strongly with the dental epithelium and weakly with the mesenchyme. MC16A16 also reacted with the cell surface of nonpermeabilized cultured dental cells and could detach epithelial cells cultured on glass, but not mesenchymal cells which maintained vinculin-containing focal contacts. This antibody, which affected the organization of dental-cell microfilaments in primary culture, also inhibited the polarization of odontoblasts, but not that of ameloblasts.

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Citations

Oct 1, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·M P MarkJ V Ruch
Mar 1, 1994·International Endodontic Journal·D Tziafas
Dec 8, 2006·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Sopee PoomsawatPaisarn Vejchapipat
Aug 15, 2012·International Endodontic Journal·P SangwanA Rohilla
Jan 1, 1994·Archives of Oral Biology·A J SmithH Lesot
Jun 7, 2011·Archives of Oral Biology·Yucheng LiJunnan Shi
May 11, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·A J Smith, H Lesot
Oct 7, 2000·Journal of Dental Research·H Lesot
Mar 19, 2003·Advances in Dental Research·M MacDougallS Chen
Mar 19, 2003·Advances in Dental Research·H LesotJ V Ruch

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