PMID: 38842Jul 19, 1979

Membrane damage by a toxin from the sea anemone Stoichactis helianthus. II. Effect of membrane lipid composition in a liposome system

Biochimica Et Biophysica Acta
M L ShinM M Mayer

Abstract

In the first paper of this series, it was shown that a toxin from the sea anemone Stoichactis helianthus increased the permeability of black lipid membranes due to transmembrane channel formation. In the present study, we have used liposomes to examine the reactivity of the toxin with different phospholipids. Membrane damage was assessed by measuring the release of 86Rb+ and 14C-labeled membrane lipid. For the different lipids, the rank order of marker release was: sphingomyelin greater than C18 : 2 phosphatidylcholine greater than C18 : 1 phosphatidylcholine greater than C18 : 0 phosphatidylcholine greater than C16 : 0 phosphatidylcholine = C14 : 0 phosphatidylcholine. In C14 : 0 and C16 : 0 phosphatidylcholine liposomes there was no 14C-labeled lipid release and only 13 to 16% 86 Rb+ release which corresponds to the 86Rb+ content in the outermost aqueous shell of multilamellar liposomes. This indicates that membrane damage was limited to the outermost bilayer. In liposomes prepared with the other lipids, the extent of release of both markers increased proportionately with the length and the degree of unsaturation of the lipids' acyl side chains. Spingomyelin liposomes were the most susceptible with 47% of the 14C-labeled lipi...Continue Reading

References

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Citations

Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A W Bernheimer, L S Avigad
Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D W MichaelsM M Mayer
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Epicholesterol
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