Membrane-damaging and cytotoxic effects on human fibroblasts of alpha- and beta-hemolysins from Aeromonas hydrophila.

Infection and Immunity
M Thelestam, A Ljungh

Abstract

The effects of two hemolysins (alpha and beta) from Aeromonas hydrophila on human lung fibroblasts were investigated. The toxins differed distinctly in regard to the morphological changes they produced. The alpha-hemolysin caused rounding of the cells. The beta-hemolysin caused a striking vacuolization of the cytoplasm in cells which remained spread out on the growth surface. The toxins also differed as to relative size of the initial lesions they induced in the fibroblast membrane, scored by leakage of different-sized cytoplasmic markers. The alpha-hemolysin induced larger lesions than did the beta-hemolysin. It was indirectly demonstrated that the alpha-hemolysin did not bind, or bound only transiently, to the fibroblasts. By contrast, the beta-hemolysin bound rapidly and firmly. The cytopathogenic response to the alpha-hemolysin was reversible, whereas cells treated with small amounts of the beta-hemolysin for only 1 min invariably died within a few hours. Thus, the two hemolysins from A. hydrophila, despite many biochemical similarities, show essential dissimilarities in their interactions with cultured cells.

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Citations

Aug 31, 2001·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·R M FalcónT Yano
Oct 27, 2011·Annals of Dermatology·Se Young ParkSeok Don Park
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Jan 1, 1982·Toxicon : Official Journal of the International Society on Toxinology·A LjunghT Wadström
Dec 1, 1988·Journal of Clinical Microbiology·H Namdari, E J Bottone

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