Membrane effects of thyrotropin-releasing hormone and estrogen shown by intracellular recording from pituitary cells

B DufyA Tixier-Vidal


The effects of thyrotropin-releasing hormone and 17 beta-estradiol on the electrical membrane properties of a prolactin-secretin pituitary cell line (GH3/B6) were studied with intracellular microelectrode recordings. Of the cells tested, 50 percent were excitable and displayed calcium-dependent action potentials when depolarized. When injected directly on the membrane of an excitable cell, thyrotropin-releasing hormone and 17 beta-estradiol induced action potentials within 1 minute. The spiking activity was preceded by a progressive increase of the input resistance without any detectable change in the resting membrane polarization. The results reveal a rapid effect of both substances on the membrane of GH3/B6 cells. In the case of thyrotropin-releasing hormone, which has both a short-term effect on release of prolactin and a long-term effect on its synthesis, the induced electrical activity may be associated with the stimulation of prolactin production. The physiological implication of 17 beta-estradiol-induced, calcium-dependent spiking activity remains to be elucidated.


Nov 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M LieberherrS Balsan
Dec 1, 1987·Pflügers Archiv : European journal of physiology·D G Lang, A K Ritchie
Feb 1, 1988·Pflügers Archiv : European journal of physiology·B HedlundL Barker
Apr 1, 1986·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·P F Copenhaver, J W Truman
Jan 1, 1985·The Journal of Membrane Biology·N YamashitaE Ogata
Jun 1, 1997·Herz·S J HutchisonK Chatterjee
Feb 1, 1980·European Journal of Pharmacology·R E Hruska, E K Silbergeld
Feb 29, 1996·European Journal of Pharmacology·C Büküşoğlu, F Sarlak
Jan 1, 1991·Life Sciences·A Martínez-CamposH A Barrera-Saldaña
Feb 1, 1982·Pharmacology, Biochemistry, and Behavior·R E HruskaE K Silbergeld
Jan 1, 1985·Neurochemistry International·J D VincentJ L Brigant
Jul 1, 1982·Molecular and Cellular Endocrinology·B DufyJ D Vincent
Nov 1, 1986·Molecular and Cellular Endocrinology·N Pourreau-SchneiderP M Martin
Apr 28, 1995·Molecular and Cellular Endocrinology·L A PivirottoP E Keeting
Sep 1, 1995·Journal of the American College of Cardiology·K SudhirK Chatterjee
Oct 1, 1995·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·D L AmininS N Fedorov
Apr 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·L GiustiA Lucacchini
Feb 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·D W BrannV B Mahesh
Apr 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·C Büküsoğlu, N R Krieger
Mar 15, 2000·Physiology & Behavior·C M ContrerasL Martínez-Mota
Apr 19, 2002·Steroids·Cheryl S WatsonB Gametchu
Jul 16, 2002·Steroids·Irina G AgafonovaSergey N Fedorov
Oct 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·G FiorelliM L Brandi
Dec 1, 1996·Trends in Endocrinology and Metabolism : TEM·S S Stojilkovic
Oct 8, 1999·Trends in Endocrinology and Metabolism : TEM·M J Kelly, E J Wagner
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S Ozawa, N Kimura
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·W H RotsztejnG Rosselin
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M AdlerF F Weight
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J M Dubinsky, G S Oxford
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J Barsony, S J Marx
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R J BorskiE G Grau
Mar 1, 1984·The Journal of General Physiology·J M Dubinsky, G S Oxford
Sep 27, 2001·Antisense & Nucleic Acid Drug Development·A H TaylorF Al-Azzawi
Dec 7, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Celeste H CampbellCheryl S Watson
Jun 11, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cheryl S WatsonMikhail Y Kochukov
May 5, 1999·Journal of Cardiovascular Pharmacology·G CeballosG Chamorro
Jun 16, 2005·Science's STKE : Signal Transduction Knowledge Environment·Kyung Hee Kim, J R Bender
Jan 1, 1997·Annual Review of Physiology·M Wehling
Mar 28, 2013·Environmental Health : a Global Access Science Source·René Viñas, Cheryl S Watson
Mar 6, 2013·Environmental Health Perspectives·René Viñas, Cheryl S Watson
Oct 1, 1995·Environmental Health Perspectives·Cheryl S WatsonB Gametchu
Nov 26, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K D Brubaker, C V Gay
Nov 17, 1982·Biochimica Et Biophysica Acta·M A BrostromC O Brostrom
Jan 1, 1984·Clinical and Experimental Hypertension. Part A, Theory and Practice·J L BarkerM A Rogawski
Apr 29, 2015·Molecular and Cellular Endocrinology·Zhao-Yi Wang, Li Yin
Apr 12, 2008·Hormones and Behavior·Sara SarkeyLydia L DonCarlos
Dec 1, 2007·Journal of Neuroendocrinology·D Chesnoy-Marchais, A Meillerais
Feb 28, 1983·Biochemical and Biophysical Research Communications·R G Canada
Oct 1, 1987·Pharmacology & Toxicology·B Hedlund
Aug 1, 1985·Clinical Endocrinology·B L BrownS Tomlinson
Dec 1, 1980·Annals of Neurology·P E Cooper, J B Martin
Feb 3, 1987·Brain Research·B Hedlund, J L Barker
May 29, 1986·Biochemical and Biophysical Research Communications·B DufyJ L Barker
Feb 9, 2000·Frontiers in Neuroendocrinology·B M SchmidtM Wehling
Oct 30, 1981·Biochemical and Biophysical Research Communications·E ZyzekJ D Vincent
Sep 2, 2006·Endocrinology·K MoriartyJ R Bender
Oct 21, 2004·American Journal of Physiology. Endocrinology and Metabolism·Nataliya N BulayevaCheryl S Watson
Jan 1, 1989·The American Journal of Physiology·M Familari, J W Funder
Dec 10, 1999·The Journal of Biological Chemistry·M TomićS S Stojilkovic

Related Concepts

Nerve Impulses
Plasma Membrane
Pituitary Stalk
Proterelin Tartrate Hydrate

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

The Tendon Seed Network

Tendons are rich in the extracellular matrix and are abundant throughout the body providing essential roles including structure and mobility. The transcriptome of tendons is being compiled to understand the micro-anatomical functioning of tendons. Discover the latest research pertaining to the Tendon Seed Network here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.


Incretins are metabolic hormones that stimulate a decrease in glucose levels in the blood and they have been implicated in glycemic regulation in the remission phase of type 1 diabetes. Here is the latest research.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Long COVID-19

“Long Covid-19” describes illness in patients who are reporting long-lasting effects of the SARS-CoV-19 infection, often long after they have recovered from acute Covid-19. Ongoing health issues often reported include low exercise tolerance and breathing difficulties, chronic tiredness, and mental health problems such as post-traumatic stress disorder and depression. This feed follows the latest research into Long Covid.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.