Membrane effects of thyrotropin-releasing hormone and estrogen shown by intracellular recording from pituitary cells

Science
B DufyA Tixier-Vidal

Abstract

The effects of thyrotropin-releasing hormone and 17 beta-estradiol on the electrical membrane properties of a prolactin-secretin pituitary cell line (GH3/B6) were studied with intracellular microelectrode recordings. Of the cells tested, 50 percent were excitable and displayed calcium-dependent action potentials when depolarized. When injected directly on the membrane of an excitable cell, thyrotropin-releasing hormone and 17 beta-estradiol induced action potentials within 1 minute. The spiking activity was preceded by a progressive increase of the input resistance without any detectable change in the resting membrane polarization. The results reveal a rapid effect of both substances on the membrane of GH3/B6 cells. In the case of thyrotropin-releasing hormone, which has both a short-term effect on release of prolactin and a long-term effect on its synthesis, the induced electrical activity may be associated with the stimulation of prolactin production. The physiological implication of 17 beta-estradiol-induced, calcium-dependent spiking activity remains to be elucidated.

Citations

Nov 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M LieberherrS Balsan
Dec 1, 1987·Pflügers Archiv : European journal of physiology·D G Lang, A K Ritchie
Feb 1, 1988·Pflügers Archiv : European journal of physiology·B HedlundL Barker
Apr 1, 1986·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·P F Copenhaver, J W Truman
Jan 1, 1985·The Journal of Membrane Biology·N YamashitaE Ogata
Jun 1, 1997·Herz·S J HutchisonK Chatterjee
Feb 1, 1980·European Journal of Pharmacology·R E Hruska, E K Silbergeld
Feb 29, 1996·European Journal of Pharmacology·C Büküşoğlu, F Sarlak
Jan 1, 1991·Life Sciences·A Martínez-CamposH A Barrera-Saldaña
Feb 1, 1982·Pharmacology, Biochemistry, and Behavior·R E HruskaE K Silbergeld
Jan 1, 1985·Neurochemistry International·J D VincentJ L Brigant
Jul 1, 1982·Molecular and Cellular Endocrinology·B DufyJ D Vincent
Nov 1, 1986·Molecular and Cellular Endocrinology·N Pourreau-SchneiderP M Martin
Apr 28, 1995·Molecular and Cellular Endocrinology·L A PivirottoP E Keeting
Sep 1, 1995·Journal of the American College of Cardiology·K SudhirK Chatterjee
Oct 1, 1995·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·D L AmininS N Fedorov
Apr 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·L GiustiA Lucacchini
Feb 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·D W BrannV B Mahesh
Apr 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·C Büküsoğlu, N R Krieger
Mar 15, 2000·Physiology & Behavior·C M ContrerasL Martínez-Mota
Apr 19, 2002·Steroids·Cheryl S WatsonB Gametchu
Jul 16, 2002·Steroids·Irina G AgafonovaSergey N Fedorov
Oct 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·G FiorelliM L Brandi
Dec 1, 1996·Trends in Endocrinology and Metabolism : TEM·S S Stojilkovic
Oct 8, 1999·Trends in Endocrinology and Metabolism : TEM·M J Kelly, E J Wagner
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S Ozawa, N Kimura
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·W H RotsztejnG Rosselin
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M AdlerF F Weight
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J M Dubinsky, G S Oxford
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J Barsony, S J Marx
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R J BorskiE G Grau
Mar 1, 1984·The Journal of General Physiology·J M Dubinsky, G S Oxford
Sep 27, 2001·Antisense & Nucleic Acid Drug Development·A H TaylorF Al-Azzawi
Dec 7, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Celeste H CampbellCheryl S Watson
Jun 11, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Cheryl S WatsonMikhail Y Kochukov
May 5, 1999·Journal of Cardiovascular Pharmacology·G CeballosG Chamorro
Jun 16, 2005·Science's STKE : Signal Transduction Knowledge Environment·Kyung Hee Kim, J R Bender
Jan 1, 1997·Annual Review of Physiology·M Wehling
Mar 28, 2013·Environmental Health : a Global Access Science Source·René Viñas, Cheryl S Watson
Mar 6, 2013·Environmental Health Perspectives·René Viñas, Cheryl S Watson
Oct 1, 1995·Environmental Health Perspectives·Cheryl S WatsonB Gametchu
Nov 26, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K D Brubaker, C V Gay
Nov 17, 1982·Biochimica Et Biophysica Acta·M A BrostromC O Brostrom
Jan 1, 1984·Clinical and Experimental Hypertension. Part A, Theory and Practice·J L BarkerM A Rogawski
Apr 29, 2015·Molecular and Cellular Endocrinology·Zhao-Yi Wang, Li Yin
Apr 12, 2008·Hormones and Behavior·Sara SarkeyLydia L DonCarlos
Dec 1, 2007·Journal of Neuroendocrinology·D Chesnoy-Marchais, A Meillerais
Feb 28, 1983·Biochemical and Biophysical Research Communications·R G Canada
Oct 1, 1987·Pharmacology & Toxicology·B Hedlund
Aug 1, 1985·Clinical Endocrinology·B L BrownS Tomlinson
Dec 1, 1980·Annals of Neurology·P E Cooper, J B Martin
Feb 3, 1987·Brain Research·B Hedlund, J L Barker
May 29, 1986·Biochemical and Biophysical Research Communications·B DufyJ L Barker
Feb 9, 2000·Frontiers in Neuroendocrinology·B M SchmidtM Wehling
Oct 30, 1981·Biochemical and Biophysical Research Communications·E ZyzekJ D Vincent
Sep 2, 2006·Endocrinology·K MoriartyJ R Bender
Oct 21, 2004·American Journal of Physiology. Endocrinology and Metabolism·Nataliya N BulayevaCheryl S Watson
Jan 1, 1989·The American Journal of Physiology·M Familari, J W Funder
Dec 10, 1999·The Journal of Biological Chemistry·M TomićS S Stojilkovic

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