Membrane electrical activity elicits inositol 1,4,5-trisphosphate-dependent slow Ca2+ signals through a Gbetagamma/phosphatidylinositol 3-kinase gamma pathway in skeletal myotubes

The Journal of Biological Chemistry
José M EltitEnrique Jaimovich

Abstract

Tetanic electrical stimulation of myotubes evokes a ryanodine receptor-related fast calcium signal, during the stimulation, followed by a phospholipase C/inositol 1,4,5-trisphosphate-dependent slow calcium signal few seconds after stimulus end. L-type calcium channels (Cav 1.1, dihydropyridine receptors) acting as voltage sensors activate an unknown signaling pathway involved in phospholipase C activation. We demonstrated that both G protein and phosphatidylinositol 3-kinase were activated by electrical stimulation, and both the inositol 1,4,5-trisphosphate rise and slow calcium signal induced by electrical stimulation were blocked by pertussis toxin, by a Gbetagamma scavenger peptide, and by phosphatidylinositol 3-kinase inhibitors. Immunofluorescence using anti-phosphatidylinositol 3-kinase gamma antibodies showed a clear location in striations within the cytoplasm, consistent with a position near the I band region of the sarcomere. The time course of phosphatidylinositol 3-kinase activation, monitored in single living cells using a pleckstrin homology domain fused to green fluorescent protein, was compatible with sequential phospholipase Cgamma1 activation as confirmed by phosphorylation assays for the enzyme. Co-transfectio...Continue Reading

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Citations

Oct 14, 2009·The Journal of Biological Chemistry·Sonja BuvinicEnrique Jaimovich
Sep 15, 2010·The Journal of General Physiology·Mariana CasasEnrique Jaimovich
Nov 19, 2011·Tissue Engineering. Part C, Methods·Alastair Khodabukus, Keith Baar
Jun 18, 2011·BMC Cancer·Erdene BaljinnyamKousaku Iwatsubo
Sep 18, 2015·Journal of Muscle Research and Cell Motility·Laszlo Csernoch, Vincent Jacquemond
May 2, 2014·Frontiers in Physiology·Luis A CeaJuan C Sáez
Sep 14, 2007·Expert Review of Medical Devices·Andrew S RowlandsJustin J Cooper-White
Jul 3, 2009·American Journal of Physiology. Cell Physiology·Gonzalo JorqueraNora Riveros
Feb 13, 2014·American Journal of Physiology. Endocrinology and Metabolism·Mario BustamanteSonja Buvinic
Jun 21, 2014·Exercise and Sport Sciences Reviews·Mariana CasasEnrique Jaimovich
Apr 17, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·César CárdenasEnrique Jaimovich
Jul 4, 2018·Materials·Megane Beldjilali-LabroCécile Legallais
Jan 17, 2013·Journal of Cell Science·Gonzalo JorqueraMariana Casas
Dec 15, 2010·Journal of Molecular and Cellular Cardiology·Matias EscobarClara Franzini-Armstrong

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