Membrane electrospray ionization for direct ultrasensitive biomarker quantitation in biofluids using mass spectrometry

Analytical Chemistry
Mei ZhangWei Xu

Abstract

The ability of rapid biomarker quantitation in raw biological samples would expand the application of mass spectrometry in clinical diagnosis. Up until now, the conventional chromatography-mass spectrometry method is time-consuming in both sample preparation and chromatography separation processes, while ambient ionization methods normally suffer from sensitivity. The membrane electrospray ionization (MESI) introduced in this study could not only achieve sensitive biomolecule quantitation, but also minimize the sample handling process. As a unique feature of MESI, both vertical and horizontal chemical separations could be achieved in real-time. With the capability of mass-selectively minimizing matrix effects from salts, small molecules, and macromolecules, ultrasensitive detection of cytochrome C (>500-fold sensitivity improvement) in raw urine samples was demonstrated in less than 20 min.

References

Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·G P DimriO Pereira-Smith
Nov 10, 2000·Journal of the American Society for Mass Spectrometry·R KingT Olah
Oct 7, 2004·Journal of the American Society for Mass Spectrometry·Keqi TangRichard D Smith
Apr 15, 2005·Analytical Chemistry·Robert B CodyH Dupont Durst
Aug 31, 2007·Journal of the American Society for Mass Spectrometry·Na NaXinrong Zhang
Nov 6, 2007·Journal of the American Society for Mass Spectrometry·Ayanna U JacksonGary J Van Berkel
Apr 18, 2008·Journal of Applied Toxicology : JAT·T J MillerJ P Hanig
Jun 28, 2008·Analytical Chemistry·Michael S WestphallLloyd M Smith
Jan 6, 2010·Angewandte Chemie·He WangZheng Ouyang
Feb 18, 2010·Analytical Chemistry·Jiangjiang LiuZheng Ouyang
Dec 18, 2010·Analytical Chemistry·Yongjing ChenPurnendu K Dasgupta
Jan 21, 2011·Analytical Chemistry·He WangZheng Ouyang
Mar 23, 2011·Analytical Chemistry·Nishant ChetwaniHsueh-Chia Chang
Oct 18, 2011·Expert Review of Proteomics·Kristin J BoggioJeffrey N Agar
Jan 17, 2012·Analytical Chemistry·Anu VaikkinenRisto Kostiainen
Mar 28, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Sachiko DateTsutomu Masujima
Apr 6, 2012·Expert Opinion on Drug Metabolism & Toxicology·David M Small, Glenda C Gobe
Feb 20, 2013·Analytical Chemistry·Bienvenida Gilbert-LópezJoachim Franzke
Mar 29, 2013·Analytical Chemistry·Xuefang ZhanYixiang Duan
May 30, 2013·Analytical Chemistry·Markus HaapalaRisto Kostiainen
Sep 17, 2013·Angewandte Chemie·Zhenwei WeiXinrong Zhang
Oct 8, 2013·Analytical Chemistry·Beixi Wang, Sarah Trimpin
Jan 16, 2014·Bioanalysis·Cheng-Huang LinTing-Yang Kuo
Jan 17, 2014·Analytical Chemistry·Yun ZhangVicki H Wysocki
Jul 23, 2014·Analytical Chemistry·Max M GongDavid Sinton
Dec 10, 2014·Analytical Chemistry·Ivan A TibavinskyAndrei G Fedorov

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Citations

Dec 10, 2015·Analytical Chemistry·German A Gomez-RiosJanusz Pawliszyn
Oct 1, 2015·Analytical Chemistry·Dalton T SnyderR Graham Cooks
Jan 6, 2016·Journal of the American Society for Mass Spectrometry·Carolina VegaNicholas E Manicke
Feb 27, 2016·Bioanalysis·Nicholas E ManickeChengsen Zhang
Aug 22, 2015·Bioanalysis·John Takyi-WilliamsKai Tang
Sep 9, 2015·Analytica Chimica Acta·Christian W Klampfl, Markus Himmelsbach
Jan 30, 2018·Journal of Analytical Toxicology·Josiah McKennaNicholas E Manicke
Jan 30, 2018·Bioanalysis·Germán Augusto Gómez-RíosJanusz Pawliszyn
Jul 18, 2017·Analytical Methods : Advancing Methods and Applications·Joshua HeinemannBrian Bothner
Sep 16, 2020·Analytical Methods : Advancing Methods and Applications·Giovanna L de AraújoBoniek G Vaz
Oct 17, 2017·Analytical and Bioanalytical Chemistry·Xianjiang LiHuwei Liu
Jan 4, 2021·Analytical and Bioanalytical Chemistry·Chaozi LiuZongxiu Nie
Mar 13, 2019·Journal of the American Society for Mass Spectrometry·William R A WichertNicholas E Manicke
Aug 2, 2017·Journal of the American Chemical Society·Chengsen ZhangNicholas E Manicke
Dec 24, 2021·Analytical Methods : Advancing Methods and Applications·Fangfang LuZhiping Zhang

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