PMID: 2110672Jan 1, 1990Paper

Membrane feeding for infection of the reduviid bug Triatoma infestans with Blastocrithidia triatomae (Trypanosomatidae) and pathogenic effects of the flagellate

Parasitology Research
G A Schaub

Abstract

To investigate the pathogenic effects of the trypanosome Blastocrithidia triatomae on Triatoma infestans, a method for direct infection by in vitro feeding was developed. After isolation of B. triatomae cysts from infected dead bugs, a suspension of cysts was disinfected with Desogen and Chloramin T, then mixed with sterile blood, spread on a sterile, parallel-grooved glass plate or a hammered aluminium plate and covered with a thin, sterile silicone membrane. The blood and plate were warmed to 36 degrees-38 degrees C by a heating plate. For investigation of the pathological effects, first instars of T. infestans were allowed to feed through the membrane on a mixture containing 10(6) cyst stages/cm3 blood. Development of about 30% of the third and fourth instar larvae was retarded as compared with that in uninfected control groups, and mortality rates increased in fourth and/or fifth instar larvae. Whereas total mortality rates of about 5% occurred in control groups, about 85% of the larvae died in the infected groups. These effects are very similar to those obtained in previous studies with coprophagic infections.

References

Oct 1, 1968·Journal of Medical Entomology·A C Pipkin, T J Connor
Jan 28, 1982·Journal of Medical Entomology·G O UbreigoM E Fontanarrosa
Jun 1, 1989·Parasitology Today·G A Schaub

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Citations

May 28, 2016·Journal of Vector Ecology : Journal of the Society for Vector Ecology·Jennifer K Peterson, Andrea L Graham
Jan 1, 1990·Parasitology Today·G A SchaubM Pudney
Nov 11, 2008·Acta Tropica·G A VallejoG A Schaub

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