Membrane fusion is induced by a distinct peptide sequence of the sea urchin fertilization protein bindin.

The Journal of Biological Chemistry
Anne S UlrichD Hoekstra

Abstract

Fertilization in the sea urchin is mediated by the membrane-associated acrosomal protein bindin, which plays a key role in the adhesion and fusion between sperm and egg. We have investigated the structure/function relationship of an 18-amino acid peptide fragment "B18," which represents the minimal membrane binding motif of the protein and resembles a putative fusion peptide. The peptide was found to mimic the behavior of its parent protein bindin with respect to (a) its high affinity for lipid bilayers, (b) the ability to aggregate and fuse vesicles, (c) the binding of Zn2+ by a histidine-rich motif, (d) the tendency to self-assemble, and (e), as indicated earlier, the adhesion to cell surface polysaccharides. Fluorescence and light scattering assays were used here to monitor peptide-induced lipid mixing, leakage, and aggregation of large unilamellar sphingomyelin/cholesterol vesicles. For these activities, B18 requires the presence of Zn2+ ions, with which it forms oligomeric complexes and assumes a partially alpha-helical conformation, as observed by circular dichroism. We conclude that aggregation and fusion involves a "trans-complex" between peptides on apposing vesicles that are connected by Zn2+ bridges.

References

Jun 28, 1991·Science·F H Arnold, B L Haymore
Apr 1, 1990·Journal of Bioenergetics and Biomembranes·D Hoekstra
Jan 19, 1990·Biochimica Et Biophysica Acta·P L DeAngelis, C G Glabe
Oct 18, 1988·Biochemistry·P L DeAngelis, C G Glabe
Jun 13, 1986·Biochimica Et Biophysica Acta·L D MayerP R Cullis
Jun 18, 1985·Biochemistry·H EllensF C Szoka
Jul 7, 1981·Biochemistry·D K StruckR E Pagano
Aug 1, 1995·Current Opinion in Biotechnology·D J Matthews
Jul 28, 1995·The Journal of Biological Chemistry·M Vidal, D Hoekstra
Jul 1, 1995·Trends in Biochemical Sciences·L Regan
Jan 1, 1993·Methods in Enzymology·N Düzgüneş, J Wilschut
Feb 9, 1993·Biochimica Et Biophysica Acta·S J Miraglia, C G Glabe
Mar 1, 1993·Developmental Biology·A LopezC G Glabe
Apr 1, 1993·Molecular Biology of the Cell·J E MinorE H Davidson
Aug 1, 1995·Current Opinion in Structural Biology·F M Hughson
Apr 12, 1996·Science·J E Rothman, F T Wieland
Oct 1, 1996·Current Opinion in Cell Biology·A P HuovilaJ M White
Jan 1, 1996·Annual Review of Cell and Developmental Biology·L D HernandezJ M White
May 1, 1997·Trends in Biochemical Sciences·R BrasseurM Rosseneu
Jul 15, 1997·Developmental Biology·R L Stears, W J Lennarz
Jun 1, 1997·The Journal of Peptide Research : Official Journal of the American Peptide Society·T NiidomeH Aoyagi

❮ Previous
Next ❯

Citations

Mar 31, 2009·Journal of Molecular Evolution·Isabel CalderónH A Lessios
Nov 8, 2006·European Biophysics Journal : EBJ·Johannes ReichertAnne S Ulrich
Nov 15, 2011·European Biophysics Journal : EBJ·Parvesh WadhwaniAnne S Ulrich
Sep 7, 2001·Cytokine & Growth Factor Reviews·A SingsonP Kadandale
Feb 13, 2009·Journal of the American Chemical Society·Rachel S Signarvic, William F Degrado
Jun 28, 2011·Integrative and Comparative Biology·Harilaos A Lessios
Nov 8, 2011·Annual Review of Physiology·Janice P Evans
Nov 28, 2007·The Journal of Cell Biology·Ankur H ShahCathleen Carlin
Sep 11, 2002·The Journal of Gene Medicine·Chantal PichonPatrick Midoux
Jan 19, 2010·Trends in Cell Biology·Julian L Wong, Mark A Johnson
Jun 21, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Keisuke NiikuraNobuhide Doi
Jul 12, 2008·Biophysical Chemistry·Hirak ChakrabortyMunna Sarkar
Jan 7, 2006·Evolution; International Journal of Organic Evolution·Kirk S ZiglerH A Lessios
Nov 25, 2003·Evolution; International Journal of Organic Evolution·Kirk S ZiglerH A Lessios
Jul 23, 2004·Evolution; International Journal of Organic Evolution·Kirk S Zigler, H A Lessios
Jan 15, 2014·Evolution; International Journal of Organic Evolution·Michael W HartChristine M Konrad
Nov 9, 2004·Chemistry and Physics of Lipids·Stephan L GrageAnne S Ulrich
May 14, 2016·BMC Genetics·Evgeniy S BalakirevFrancisco J Ayala
Nov 13, 2003·Chembiochem : a European Journal of Chemical Biology·Sergii AfoninAnne S Ulrich
Aug 9, 2006·Molecular Reproduction and Development·Ana Vieira, David J Miller
Sep 21, 2006·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Sergii AfoninAnne S Ulrich
Nov 13, 2007·Journal of Peptide Science : an Official Publication of the European Peptide Society·Sandra RochaGerald Brezesinski
Feb 14, 2004·Molecular Biology and Evolution·Michael A McCartney, H A Lessios
Jan 28, 2004·Magnetic Resonance in Chemistry : MRC·Sergii AfoninAnne S Ulrich
Oct 9, 2002·The Journal of Biological Chemistry·Yuichi Takeda, Kunihiro Kasamo
Apr 15, 2011·Biology of Reproduction·Ryuzo Yanagimachi
Nov 9, 2006·Journal of the American Chemical Society·Yun GongDennis Bong
Dec 4, 2009·The Journal of Physical Chemistry. B·Sutapa Mondal, Munna Sarkar
Mar 30, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Sandra RochaGerald Brezesinski
Sep 25, 1998·Science·V D Vacquier

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.