PMID: 3771641Oct 1, 1986Paper

Membrane glycoproteins of the nerve growth cone: diversity and growth regulation of oligosaccharides

The Journal of Cell Biology
L M Greenberger, K H Pfenninger

Abstract

A subcellular fraction prepared from fetal rat brain and enriched in growth cone membranes is analyzed for its lectin-binding proteins. Growth-associated glycoproteins are identified by comparing the growth cone glycoproteins with those of synaptosomes. Protein was resolved in one- or two-dimensional gels, electroblotted, and blots probed with radioiodinated concanavalin A, wheat germ agglutinin, and Ricinus communis agglutinins I and II. In one-dimensional gels, each lectin recognizes approximately 20 polypeptides (with substantial overlap) most of which migrate diffusely and have relatively high molecular masses (range 30-200 kD). The seven major Coomassie-staining proteins of the membrane fraction (34-52 kD) are not the major lectin-binding proteins. In two-dimensional gels, the lectin-binding proteins are either streaked across the pH gradient or exist as multiple spots, indicating broad charge heterogeneity. Seven wheat germ agglutinin- and Ricinus communis agglutinin II-binding glycoproteins are present in greater abundance in growth cone fractions compared with synaptosomes. Most notably, an acidic, sialic acid-rich protein (27-30 kD, pI 4.0; termed gp27-30) is most abundant at postnatal day 4, but absent from adult brai...Continue Reading

References

Jan 1, 1977·Cold Spring Harbor Symposia on Quantitative Biology·A F WilliamsR J Morris
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1979·Neuroscience·M E HattenR L Sidman
Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K Burridge
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·W B Stallcup, L Beasley
Nov 1, 1985·The Journal of Cell Biology·I WallisK H Pfenninger
Jul 1, 1969·Analytical Biochemistry·R M ZachariusJ J Woodlock
Jun 1, 1973·Analytical Biochemistry·J H ShaperR L Hill
Nov 15, 1981·Analytical Biochemistry·W WrayR Hancock
May 14, 1982·Science·A F Williams, J Gagnon
Feb 1, 1983·Analytical Biochemistry·W Lin, H Kasamatsu
May 1, 1983·Analytical Biochemistry·J M Gershoni, G E Palade
Aug 1, 1983·Analytical Biochemistry·K Hancock, V C Tsang
Jan 1, 1982·Annual Review of Biochemistry·G Ashwell, J Harford
Jan 1, 1983·Annual Review of Physiology·S C Landis
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B A CunninghamG M Edelman
Jan 1, 1984·The Journal of Cell Biology·P SondereggerP G Nelson
Jan 1, 1982·Analytical Biochemistry·R HawkesJ Gordon
Jun 1, 1981·The Journal of Cell Biology·K H Pfenninger, M F Maylié-Pfenninger

❮ Previous
Next ❯

Citations

May 1, 1990·Brain Research. Brain Research Reviews·R O Lockerbie
Oct 1, 1990·Brain Research. Developmental Brain Research·B T EdmondsL Ellis
Mar 1, 1991·The Journal of Cell Biology·R O LockerbieK H Pfenninger
Sep 1, 1992·Journal of Neurobiology·S H HanissianN E Sahyoun
Jul 1, 1988·Journal of Neurochemistry·M Sbaschnig-AglerR W Ledeen
Oct 1, 1992·Neurochemical Research·N Ishioka, S Kurioka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.