Membrane insertion of the three main membranotropic sequences from SARS-CoV S2 glycoprotein.

Biochimica Et Biophysica Acta
Jaime GuillénJosé Villalaín

Abstract

In order to complete the fusion process of SARS-CoV virus, several regions of the S2 virus envelope glycoprotein are necessary. Recent studies have identified three membrane-active regions in the S2 domain of SARS-CoV glycoprotein, one situated downstream of the minimum furin cleavage, which is considered the fusion peptide (SARSFP), an internal fusion peptide located immediately upstream of the HR1 region (SARSIFP) and the pre-transmembrane domain (SARSPTM). We have explored the capacity of these selected membrane-interacting regions of the S2 SARS-CoV fusion protein, alone or in equimolar mixtures, to insert into the membrane as well as to perturb the dipole potential of the bilayer. We show that the three peptides interact with lipid membranes depending on lipid composition and experiments using equimolar mixtures of these peptides show that different segments of the protein may act in a synergistic way suggesting that several membrane-active regions could participate in the fusion process of the SARS-CoV.

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Citations

Jul 21, 2012·Viruses·Sandrine BelouzardGary R Whittaker
Aug 14, 2012·Biochimica Et Biophysica Acta·F Palomares-JerezJosé Villalaín
Nov 29, 2016·Scientific Reports·Luis G M BassoAntonio J Costa-Filho
Apr 24, 2019·The Journal of Membrane Biology·Geetanjali Meher, Hirak Chakraborty
Feb 25, 2021·Computational and Structural Biotechnology Journal·Wai-Yim ChingRudolf Podgornik
Mar 5, 2021·Journal of Structural Biology·Debnath Pal
Mar 11, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Gourab Prasad Pattnaik, Hirak Chakraborty
Mar 7, 2021·Biophysical Journal·Defne GorgunEmad Tajkhorshid
Oct 11, 2017·Biochimica Et Biophysica Acta. Biomembranes·Mukesh MahajanSurajit Bhattacharjya
Jun 15, 2021·Journal of Medical Virology·Chaogeng ZhuWei Xu
Jan 9, 2021·Journal of Chemical Information and Modeling·Subhomoi BorkotokyManidipa Banerjee

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Methods Mentioned

BETA
acetylation
amidation

Software Mentioned

FilmWare

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