Membrane insertion pathway of annexin B12: thermodynamic and kinetic characterization by fluorescence correlation spectroscopy and fluorescence quenching.

Biochemistry
Yevgen O PosokhovAlexey S Ladokhin

Abstract

Experimental determination of the free energy stabilizing the structure of membrane proteins in their native lipid environment is undermined by the lack of appropriate methods and suitable model systems. Annexin B12 (ANX) is a soluble protein which reversibly inserts into lipid membranes under mildly acidic conditions, which makes it a good experimental model for thermodynamic studies of folding and stability of membrane proteins. Here we apply fluorescence correlation spectroscopy for quantitative analysis of ANX partitioning into large unilamellar vesicles containing either 25% or 75% anionic lipids. Membrane binding of ANX results in changes of autocorrelation time and amplitude, both of which are used in quantitative analysis. The thermodynamic scheme describing acid-induced membrane interactions of ANX considers two independent processes: pH-dependent formation of a membrane-competent form near the membrane interface and its insertion into the lipid bilayer. Our novel fluorescence lifetime topology method demonstrates that the insertion proceeds via an interfacial refolded intermediate state, which can be stabilized by anionic lipids. Lipid titration measurements are used to determine the free energy of both transmembrane ...Continue Reading

References

Sep 24, 1991·Biochemistry·J Seelig, P Ganz
Jun 13, 1986·Biochimica Et Biophysica Acta·L D MayerP R Cullis
Jan 1, 1994·Annual Review of Biophysics and Biomolecular Structure·M A Swairjo, B A Seaton
Oct 1, 1996·Nature Structural Biology·W C Wimley, S H White
May 22, 1998·Journal of Molecular Biology·W C WimleyS H White
Sep 29, 1998·Methods in Enzymology·S H WhiteK Hristova
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·R LangenH T Haigler
Jan 26, 1999·Journal of Molecular Biology·A S Ladokhin, S H White
Sep 12, 2000·Current Opinion in Structural Biology·C R Sanders, J K Nagy
Jul 4, 2001·The Journal of Biological Chemistry·S H WhiteK Hristova
Oct 3, 2001·Journal of Molecular Biology·S JayasingheS H White
Jan 25, 2002·Nature·Michael Zasloff
Sep 11, 2002·Biochemistry·Chang Sup KimYeon-Kyun Shin
Dec 20, 2002·Biopolymers·Yechiel Shai
Dec 20, 2002·Biopolymers·Lukas K TammAlex L Lai
Feb 28, 2003·Methods : a Companion to Methods in Enzymology·Elke Haustein, Petra Schwille
May 12, 2004·Biochemistry·Alexey S Ladokhin, Stephen H White
Jun 9, 2004·Biochemistry·Alexey S LadokhinStephen H White
Jul 9, 2004·Nature·Maria L Garcia
Jan 28, 2005·Nature·James U Bowie
Nov 22, 2005·Analytical Biochemistry·Yevgen O Posokhov, Alexey S Ladokhin
Dec 2, 2005·Nature·Stuart McLaughlin, Diana Murray
Dec 6, 2006·Biochemistry·Robert Renthal
May 29, 2007·Journal of Molecular Biology·Mónica Fernández-VidalStephen H White
Jun 19, 2007·Biophysical Journal·Yevgen O PosokhovAlexey S Ladokhin
Jun 23, 2007·The Journal of Biological Chemistry·Aurélie PerierDaniel Gillet

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Citations

Jun 7, 2011·Biochemistry. Biokhimii︠a︡·I V PerevoshchikovaY N Antonenko
Aug 27, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Robert GodinGonzalo Cosa
Apr 30, 2013·Biochemistry·Mauricio Vargas-UribeAlexey S Ladokhin
Oct 1, 2011·The Journal of Physical Chemistry. B·Hanna EngelkeJoachim O Rädler
Aug 20, 2014·Biochimica Et Biophysica Acta·Kasper KristensenThomas L Andresen
Sep 8, 2015·Biochimica Et Biophysica Acta·Franziska A ThomasPetra Schwille
Feb 5, 2015·The Journal of Membrane Biology·Jose C Flores-CanalesMaria Kurnikova
Oct 5, 2014·The Journal of Membrane Biology·Mauricio Vargas-UribeAlexey S Ladokhin
Feb 11, 2014·Biophysical Journal·Alexander KyrychenkoAlexey S Ladokhin
Aug 28, 2012·Journal of Molecular Biology·Vanessa C Ducas, Elizabeth Rhoades
Jan 10, 2012·Journal of Molecular Biology·Alexander KyrychenkoAlexey S Ladokhin
Oct 1, 2015·Methods and Applications in Fluorescence·Alexander Kyrychenko
Oct 15, 2019·Frontiers in Molecular Biosciences·H RaghuramanAnindita Das
Feb 11, 2021·The Journal of Membrane Biology·Victor Vasquez-Montes, Alexey S Ladokhin
Apr 21, 2019·Biochimica Et Biophysica Acta. Proteins and Proteomics·Victor Vasquez-MontesAlexey S Ladokhin
Dec 20, 2008·Analytical Chemistry·Alexei TcherniakChristy F Landes

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