Membrane interactions and self-association of components of the Ess/Type VII secretion system of Staphylococcus aureus

FEBS Letters
Franziska JägerTracy Palmer

Abstract

The Ess/Type VII protein secretion system, essential for virulence of pathogenic Staphylococcus aureus, is dependent upon the four core membrane proteins EssA, EssB, EssC and EsaA. Here, we use crosslinking and blue native PAGE analysis to show that the EssB, EssC and EsaA proteins individually form homomeric complexes. Surprisingly, these components appear unable to interact with each other, or with the EssA protein. We further show that two high molecular weight multimers of EssC detected in whole cells are not dependent upon the presence of EsxA, EsxB or any other Ess component for their assembly.

References

Jan 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Monica L BurtsDominique M Missiakas
Feb 17, 2006·The Journal of Biological Chemistry·Carlos São-JoséPaulo Tavares
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May 16, 2015·Nature Reviews. Microbiology·Tiago R D CostaGabriel Waksman

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Citations

Sep 27, 2016·Nature Reviews. Microbiology·Matthias I GröschelRoland Brosch
Apr 6, 2018·Microbiology·Franziska JägerTracy Palmer
Sep 7, 2017·Journal of Bacteriology·Khaled A AlyDominique Missiakas
Aug 9, 2017·Frontiers in Cellular and Infection Microbiology·Melania CrucianiEliana M Coccia
May 1, 2016·The Biochemical Journal·Martin ZoltnerWilliam N Hunter
Aug 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Fatima R UlhuqTracy Palmer
May 28, 2021·Nature Reviews. Microbiology·Angel Rivera-CalzadaSebastian Geibel
Aug 11, 2019·Microbiology Spectrum·Vincent J C van WindenMiriam Braunstein
Aug 4, 2021·Annual Review of Microbiology·Lisa Bowman, Tracy Palmer

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Methods Mentioned

BETA
electrophoresis

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