Membrane lipid biosynthesis in Chlamydomonas reinhardtii: ethanolaminephosphotransferase is capable of synthesizing both phosphatidylcholine and phosphatidylethanolamine

Archives of Biochemistry and Biophysics
Wenyu YangT S Moore

Abstract

Phosphatidylethanolamine, but not phosphatidylcholine, is found in Chlamydomonas reinhardtii. A cDNA coding for diacylglycerol: CDP-ethanolamine ethanolaminephosphotransferase (EPT) was cloned from C. reinhardtii. The C. reinhardtii EPT appears phylogenetically more similar to mammalian aminoalcoholphosphotransferases than to those of yeast and the least close to those of plants. Similar membrane topography was found between the C. reinhardtii EPT and the aminoalcoholphosphotransferases from mammals, yeast, and plants. A yeast mutant deficient in both cholinephosphotransferase and ethanolaminephosphotransferase was complemented by the C. reinhardtii EPT gene. Enzymatic assays of C. reinhardtii EPT from the complemented yeast microsomes demonstrated that the C. reinhardtii EPT synthesized both PC and PE in the transformed yeast. The addition of either unlabeled CDP-ethanolamine or CDP-choline to reactions reduced incorporation of radiolabeled CDP-choline and radiolabeled CDP-ethanolamine into phosphatidylcholine and phosphatidylethanolamine. EPT activity from the transformed yeast or C. reinhardtii cells was inhibited nearly identically by unlabeled CDP-choline, CDP-ethanolamine, and CMP when [14C]CDP-choline was used as the pri...Continue Reading

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Citations

Aug 17, 2010·Biochimie·Inna Khozin-Goldberg, Zvi Cohen
May 22, 2007·Progress in Lipid Research·Athanasios Lykidis
Feb 24, 2006·Progress in Lipid Research·Irina A Guschina, John L Harwood
Mar 19, 2014·Biochimica Et Biophysica Acta·Andreia F Verissimo, Fevzi Daldal
Mar 27, 2018·Rapid Communications in Mass Spectrometry : RCM·Ansgar KorfHeiko Hayen
Feb 11, 2015·The Plant Journal : for Cell and Molecular Biology·Yonghua Li-BeissonWayne Riekhof
May 10, 2017·Biochemical and Biophysical Research Communications·Takashi HirashimaNaoki Sato
Feb 1, 2019·Progress in Lipid Research·Yonghua Li-BeissonJohn L Harwood

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