Membrane lipid domains and rafts: current applications of fluorescence lifetime spectroscopy and imaging

Chemistry and Physics of Lipids
Rodrigo F M de AlmeidaManuel Prieto

Abstract

Membrane microdomains and their involvement in cellular processes are part of the current paradigm of biomembranes. However, a better characterization of domains, namely lipid rafts, is needed. In this review, it is shown how the use of time-resolved fluorescence, with the adequate parameters and probes, helps elucidating the type, number, fraction, composition and size of lipid phases and domains in multicomponent model systems. The determination of phase diagrams for lipid mixtures containing sphingolipids and/or cholesterol is exemplified. The use of fluorescence quenching and Förster resonance energy transfer (FRET) are also illustrated. Strategies for studying protein-induced domains are presented. The advantages of using single point microscopic decays and fluorescence lifetime imaging microscopy (FLIM) in systems with three-phase coexistence are explained. Finally, the introduction of FLIM allows studies in live cell membranes, and the nature of the microdomains observed is readily elucidated due to the information retrieved from fluorescence lifetimes.

References

Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M B Sankaram, T E Thompson
Nov 27, 1990·Biochemistry·M B Sankaram, T E Thompson
Aug 23, 1988·Biochemistry·K Simons, G van Meer
Nov 27, 1987·Biochimica Et Biophysica Acta·J H IpsenM J Zuckermann
Sep 3, 1993·Science·M S Bretscher, S Munro
Jul 17, 1998·Biochimica Et Biophysica Acta·R Koynova, M Caffrey
Mar 4, 1999·Biophysical Chemistry·B Ravichandra, P G Joshi
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·J KorlachG W Feigenson
Feb 27, 2001·Biophysical Journal·C DietrichE Gratton
Apr 5, 2001·Biochimica Et Biophysica Acta·L M LouraM Prieto
Jan 25, 2002·Biophysical Journal·Rodrigo F M de AlmeidaManuel Prieto
Mar 9, 2002·The Journal of Biological Chemistry·Amanda J CarozziRobert G Parton
Jan 16, 2003·The Journal of Cell Biology·Ian A PriorJohn F Hancock
Apr 5, 2003·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Peter P Ruvolo
May 3, 2003·Nature Reviews. Molecular Cell Biology·Michael Edidin
Jul 26, 2003·Journal of Biomedical Optics·Enrico GrattonNicholas Barry
Sep 26, 2003·Biophysical Journal·Rodrigo F M de AlmeidaManuel Prieto
Sep 26, 2003·Biophysical Journal·Fábio FernandesMarcus A Hemminga

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Citations

Oct 19, 2010·European Biophysics Journal : EBJ·Stanley W BotchwayChristopher D Stubbs
Apr 19, 2013·Journal of Chemical Biology·María GarridoL-Ruth Montes
Apr 2, 2010·Chemical Reviews·Mikhail Y Berezin, Samuel Achilefu
Mar 12, 2010·Journal of Agricultural and Food Chemistry·Sophie GallierDavid Everett
Apr 15, 2011·Cell Death & Disease·Y LiuW Jia
Nov 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Gwendolyn Barceló-CoblijnPablo V Escribá
Mar 3, 2010·Annual Review of Biophysics·Elliot L ElsonGuy M Genin
Jun 18, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ilse Delint-RamirezSeth G N Grant
Dec 4, 2012·International Journal of Molecular Sciences·Luís M S Loura
Jun 3, 2011·World Journal of Gastroenterology : WJG·Angela Dolganiuc
Nov 13, 2013·Chemistry and Physics of Lipids·Miroslava Dékány FraňováIlpo Vattulainen
Oct 21, 2015·European Biophysics Journal : EBJ·Filip SchröterKarin Müller
Aug 2, 2015·Chemistry and Physics of Lipids·Manuela DenzPeter Müller
Nov 13, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Cátia BandeirasBenilde Saramago
May 5, 2012·Progress in Lipid Research·Jean-Luc CacasSébastien Mongrand
Mar 28, 2012·Developmental and Comparative Immunology·Erick Garcia-GarciaMiodrag Belosevic
Jun 15, 2010·Journal of Photochemistry and Photobiology. B, Biology·Carlos A de OliveiraFrancisco B T Pessine
Feb 13, 2010·Cell Calcium·Luis Vaca
Aug 12, 2014·Journal of Dairy Science·Haotian ZhengDavid W Everett
Jul 21, 2015·Bioelectrochemistry·Lucia Becucci, Rolando Guidelli
Jan 31, 2013·Biochimica Et Biophysica Acta·Maria Laura MartinPablo V Escribá
Dec 8, 2010·Biochimica Et Biophysica Acta·Avigdor Leftin, Michael F Brown
Dec 23, 2009·Biochimica Et Biophysica Acta·Anna Celli, Enrico Gratton
Dec 1, 2012·Biochimica Et Biophysica Acta·Ana R P VarelaLiana C Silva
Oct 20, 2010·Biochimica Et Biophysica Acta·Joaquim T MarquêsRodrigo F M De Almeida
Jan 9, 2010·Biochimica Et Biophysica Acta·M T Stöckl, A Herrmann
Jan 12, 2010·Biochimica Et Biophysica Acta·Derek Marsh
Dec 15, 2010·Physical Chemistry Chemical Physics : PCCP·Hari S MuddanaPeter J Butler
Jan 1, 2014·The Journal of Physical Chemistry. B·Iwan Setiawan, G J Blanchard
Feb 5, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Alena KhmelinskaiaPablo V Escribá
Apr 3, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Joseph LorentMarie-Paule Mingeot-Leclercq
Oct 23, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·A Sheila Holmes-SmithGraham Hungerford

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