Membrane lipid metabolism and phospholipase activity in insect Spodoptera frugiperda 9 ovarian cells

Lipids
L H YehG Y Sun

Abstract

Although there is increasing use of insect ovarian Sf9 cells for the production of recombinant proteins, namely, via the baculovirus vector expression system, little is known about the lipids in the cell membrane and whether endogenous phospholipases are present for regulation of the cell membrane lipids. In this study, analysis of membrane lipids of Sf9 cells indicated the presence of phosphatidylethanolamine (PE) (diacyl type) and phosphatidylcholine as major phospholipids, followed by phosphatidylserine and phosphatidylinositol (PI), and only trace amounts of ethanolamine plasmalogen. These phospholipids contain high proportions of monoenoic fatty acids, e.g., 16:1 and 18:1, which comprise more than 70% of the total fatty acids although small amounts of polyunsaturated fatty acids such as 18:2 and 20:4 are also present. When Sf9 cells were incubated in a culture medium containing [14C]oleic acid and [14C]arachidonic acid, a large portion of the labels were incorporated into membrane phospholipids. Using [14C]arachidonoyl-phospholipids as substrates for incubation with cell homogenate and subcellular fractions, results indicate the presence of a ca(2+)-independent phospholipase A (PLA2) in the Sf9 cell cytosol fraction. This ...Continue Reading

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E H RadanyC F Deschepper
Feb 1, 1990·Journal of Neurochemistry·J M Wierenga, R M Hollingworth
Oct 14, 1988·Biochimica Et Biophysica Acta·A J PierikH Van den Bosch
Jan 1, 1987·Annual Review of Biochemistry·M J Berridge
Nov 16, 1995·Biochimica Et Biophysica Acta·E J Ackermann, E A Dennis
Jan 1, 1993·Archives of Insect Biochemistry and Physiology·J M Uscian, D W Stanley-Samuelson
Nov 1, 1993·Archives of Biochemistry and Biophysics·M S de CarvalhoC C Leslie
Jan 28, 1993·Nature·M J Berridge
Dec 1, 1995·European Journal of Biochemistry·O WeiergräberS Stabel
Jan 1, 1996·International Review of Cytology·J J Vanden Broeck

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Citations

Apr 25, 2000·Archives of Biochemistry and Biophysics·J F GreeneB D Hammock
Nov 17, 2012·Insect Biochemistry and Molecular Biology·Miriam OlombradaLucía García-Ortega
Jun 6, 2000·Biochimica Et Biophysica Acta·A R SiddiqiC C Leslie
Jun 25, 2004·The Journal of Biological Chemistry·Ikuo HayashiTakeshi Iwatsubo
Mar 16, 2000·In Vitro Cellular & Developmental Biology. Animal·M GerbalM Duonor-Cerutti
Apr 17, 2020·Biochimica Et Biophysica Acta. Biomembranes·Maruša NovakKristina Sepčić

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