PMID: 6111342Feb 20, 1981Paper

Membrane localization of myocardial type II cyclic AMP-dependent protein kinase activity

Biochimica Et Biophysica Acta
L R JonesD R Hathaway

Abstract

Crude cardiac membrane vesicles were separated into subfractions of sarcolemma and sarcoplasmic reticulum. The subfractions were used to determine the origin and type of cyclic AMP-dependent protein kinase activity present in myocardial membranes. A cyclic AMP-binding protein of molecular weight 55,000 was covalently labeled with the photoaffinity probe 8-azido adenosine 3',5'-mono[32P]phosphate, and found to copurify with the (Na+ + K+)-ATPase activity of sarcolemma, and away from the (Ca2+ + K+)-ATPase activity of sarcoplasmic reticulum. Endogenous cyclic AMP-dependent protein kinase activity also copurified with sarcolemma. Protein substrates phosphorylated by cyclic AMP-dependent protein kinase activity had apparent molecular weights of 21,000 and 8000 and were present in both sarcolemma and sarcoplasmic reticulum. However, while addition of cyclic AMP alone resulted in phosphorylation of sarcolemma proteins, both cyclic AMP and exogenous, soluble cyclic AMP-dependent kinase were required for phosphorylation of sarcoplasmic reticulum proteins. Addition of the calcium-binding protein, calmodulin, to either sarcolemma or sarcoplasmic reticulum resulted in phosphorylation of the 21,000 and 8000-dalton proteins, as well. The re...Continue Reading

References

May 17, 1979·Biochimica Et Biophysica Acta·H J MisselwitzA Wollenberger
Nov 1, 1979·Archives of Biochemistry and Biophysics·P J St Louis, P V Sulakhe
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Citations

May 1, 1982·Journal of Molecular and Cellular Cardiology·M L RinaldiJ G Demaille
May 16, 1983·European Journal of Biochemistry·P Caroni, E Carafoli
Apr 1, 1986·Archives of Biochemistry and Biophysics·C F LouisJ Turnquist
Mar 1, 1984·Circulation Research·J M LamersT J Ruigrok
Jul 1, 1982·European Journal of Biochemistry·D Toru-DelbauffeM Pavlovic-Hournac
Dec 24, 2005·American Journal of Physiology. Cell Physiology·Kristan L LansberyRobert W Mercer
Jul 5, 1983·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J Haiech, J G Demaille
Oct 28, 1998·Physiological Reviews·H K Simmerman, L R Jones
Sep 1, 1985·The American Journal of Physiology·D R Hathaway, J R Haeberle
Oct 21, 2020·Pflügers Archiv : European journal of physiology·Jana GaburjakovaMarta Gaburjakova
Jun 11, 1999·Biochemical and Biophysical Research Communications·S E Cala

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