Abstract
Crude cardiac membrane vesicles were separated into subfractions of sarcolemma and sarcoplasmic reticulum. The subfractions were used to determine the origin and type of cyclic AMP-dependent protein kinase activity present in myocardial membranes. A cyclic AMP-binding protein of molecular weight 55,000 was covalently labeled with the photoaffinity probe 8-azido adenosine 3',5'-mono[32P]phosphate, and found to copurify with the (Na+ + K+)-ATPase activity of sarcolemma, and away from the (Ca2+ + K+)-ATPase activity of sarcoplasmic reticulum. Endogenous cyclic AMP-dependent protein kinase activity also copurified with sarcolemma. Protein substrates phosphorylated by cyclic AMP-dependent protein kinase activity had apparent molecular weights of 21,000 and 8000 and were present in both sarcolemma and sarcoplasmic reticulum. However, while addition of cyclic AMP alone resulted in phosphorylation of sarcolemma proteins, both cyclic AMP and exogenous, soluble cyclic AMP-dependent kinase were required for phosphorylation of sarcoplasmic reticulum proteins. Addition of the calcium-binding protein, calmodulin, to either sarcolemma or sarcoplasmic reticulum resulted in phosphorylation of the 21,000 and 8000-dalton proteins, as well. The re...Continue Reading
References
Mar 8, 1977·Biochemistry·C B Klee
Oct 19, 1979·Biochimica Et Biophysica Acta·L R Jones
Apr 1, 1976·Archives of Biochemistry and Biophysics·C W HuiG I Drummond
Sep 14, 1977·Biochimica Et Biophysica Acta·H L Wray, R R Gray
Dec 19, 1978·Biochimica Et Biophysica Acta·L R JonesH R Besch
May 17, 1979·Biochimica Et Biophysica Acta·H J MisselwitzA Wollenberger
Nov 13, 1979·Biochemistry·C J Le PeuchJ G Demaille
Nov 1, 1979·Archives of Biochemistry and Biophysics·P J St Louis, P V Sulakhe
Oct 3, 1978·Biochimica Et Biophysica Acta·H WillA Wollenberger
May 1, 1978·Analytical Biochemistry·R Swanstrom, P R Shank
Jan 25, 1977·Biochimica Et Biophysica Acta·M A Porzio, A M Pearson
Jun 22, 1971·Biochemistry·G FairbanksD F Wallach
Dec 1, 1972·Journal of Molecular and Cellular Cardiology·M A KirchbergerA M Katz
Dec 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·J F Kuo, P Greengard
Feb 27, 1980·Biochemical and Biophysical Research Communications·E G KraniasA Schwartz
Oct 1, 1980·Biochimica Et Biophysica Acta·J M Bidlack, A E Shamoo
Citations
Nov 19, 1989·Molecular and Cellular Biochemistry·R VetterN S Dhalla
Aug 1, 1983·Journal of Bioenergetics and Biomembranes·F MandelA Schwartz
Jan 1, 1991·Archives of Toxicology·P R KodavantiD Desaiah
Jan 1, 1983·The International Journal of Biochemistry·J VelemaJ Zaagsma
May 1, 1982·Journal of Molecular and Cellular Cardiology·M L RinaldiJ G Demaille
Sep 1, 1990·Pharmacology & Toxicology·P R KodavantiD Desaiah
Apr 1, 1988·Archives of Biochemistry and Biophysics·S I WalaasA Adler
May 16, 1983·European Journal of Biochemistry·P Caroni, E Carafoli
Apr 1, 1986·Archives of Biochemistry and Biophysics·C F LouisJ Turnquist
Oct 1, 1988·Forensic Science International : Synergy·Y Iwasa, T Onaya
Jun 21, 1996·The Journal of Biological Chemistry·L G ReddyD L Stokes
Mar 1, 1984·Circulation Research·J M LamersT J Ruigrok
Mar 5, 1998·Circulation Research·Z ChenS E Cala
Jul 1, 1982·European Journal of Biochemistry·D Toru-DelbauffeM Pavlovic-Hournac
Mar 31, 2005·The Journal of Membrane Biology·C E KellyS E Cala
Jun 18, 2005·Journal of Applied Physiology·Justin ReisYuk-Chow Ng
Dec 24, 2005·American Journal of Physiology. Cell Physiology·Kristan L LansberyRobert W Mercer
Jul 5, 1983·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J Haiech, J G Demaille
Apr 24, 2003·The Journal of Biological Chemistry·Mary L RuehrMeredith Bond
Oct 28, 1998·Physiological Reviews·H K Simmerman, L R Jones
Sep 1, 1985·The American Journal of Physiology·D R Hathaway, J R Haeberle
Oct 21, 2020·Pflügers Archiv : European journal of physiology·Jana GaburjakovaMarta Gaburjakova
Jun 11, 1999·Biochemical and Biophysical Research Communications·S E Cala
Feb 15, 1988·Biochemical Pharmacology·Y SasakiS Takeyama
Dec 6, 1982·Biochimica Et Biophysica Acta·E G KraniasR A Jungmann
Feb 9, 1983·Biochimica Et Biophysica Acta·J P CaponyJ G Demaille
Nov 28, 1983·Biochimica Et Biophysica Acta·E G KraniasA Schwartz
Aug 31, 1989·Biochimica Et Biophysica Acta·H K SimmermanL R Jones
Apr 1, 1982·American Heart Journal·J P LindemannA M Watanabe