Membrane localization of scaffold proteins promotes graded signaling in the yeast MAP kinase cascade.

Current Biology : CB
Satoe Takahashi, Peter M Pryciak

Abstract

Signaling through mitogen-activated protein kinase (MAPK) cascade pathways can show various input-output behaviors, including either switch-like or graded responses to increasing levels of stimulus. Prior studies suggest that switch-like behavior is promoted by positive feedback loops and nonprocessive phosphorylation reactions, but it is unclear whether graded signaling is a default behavior or whether it must be enforced by separate mechanisms. It has been hypothesized that scaffold proteins promote graded behavior. Here, we experimentally probe the determinants of graded signaling in the yeast mating MAPK pathway. We find that graded behavior is robust in that it resists perturbation by loss of several negative-feedback regulators. However, the pathway becomes switch-like when activated by a crosstalk stimulus that bypasses multiple upstream components. To dissect the contributing factors, we developed a method for gradually varying the signal input at different pathway steps in vivo. Input at the beginning of the kinase cascade produced a sharp, threshold-like response. Surprisingly, the scaffold protein Ste5 increased this threshold behavior when limited to the cytosol. However, signaling remained graded whenever Ste5 was ...Continue Reading

References

Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·C Y Huang, J E Ferrell
Aug 1, 1997·Trends in Biochemical Sciences·J E Ferrell
Mar 14, 2000·Current Opinion in Cell Biology·W R Burack, A S Shaw
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·A LevchenkoP W Sternberg
Jun 8, 2001·Annual Review of Biochemistry·H G Dohlman, J W Thorner
Dec 26, 2001·Science's STKE : Signal Transduction Knowledge Environment·J E Ferrell
Jan 10, 2002·Nature Cell Biology·F van DrogenM Peter
Feb 21, 2003·Current Biology : CB·Christoph P BagowskiJames E Ferrell
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Deborah K Morrison, Roger J Davis
Aug 10, 2004·Yeast·Bente Kofahl, Edda Klipp
May 19, 2005·Molecular and Cellular Biology·Jeffrey P MackeiganJohn Blenis
Aug 18, 2005·Journal of Cell Science·Maosong Qi, Elaine A Elion
Sep 20, 2005·Nature·Alejandro Colman-LernerRoger Brent
Oct 18, 2005·Nature Reviews. Molecular Cell Biology·Walter Kolch
Feb 17, 2006·Nature Reviews. Molecular Cell Biology·Boris N Kholodenko
Jan 30, 2007·Nature Genetics·Megan N McCleanSharad Ramanathan
Feb 21, 2007·Nature·Saurabh PaliwalAndre Levchenko
Apr 3, 2007·Current Biology : CB·Nicholas T Ingolia, Andrew W Murray
Jul 10, 2007·Nature Cell Biology·Tianhai TianJohn F Hancock
Jul 24, 2007·Cellular Signalling·Parag Patwardhan, W Todd Miller
Sep 19, 2007·Methods in Enzymology·Stefan HohmannBodil Nordlander
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Brian D SlaughterRong Li
Jun 10, 2008·Molecular Cell·Nan HaoHenrik G Dohlman

❮ Previous
Next ❯

Citations

May 25, 2010·Nature Reviews. Molecular Cell Biology·Boris N KholodenkoWalter Kolch
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Buyong Ma, Ruth Nussinov
Jul 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kazuhiro AokiMichiyuki Matsuda
Apr 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Tetsuya MuramotoJonathan R Chubb
Aug 25, 2012·Molecular Biology of the Cell·Hsin ChenDaniel J Lew
Apr 14, 2010·Nucleic Acids Research·Raik Grünberg, Luis Serrano
Apr 17, 2010·Science·Sergio G PeisajovichWendell A Lim
Dec 15, 2011·BMC Systems Biology·Ching-Shan ChouTau-Mu Yi
Mar 23, 2010·PLoS Biology·János Z KelemenAttila Becskei
Jul 30, 2010·PloS One·Craig J Thalhauser, Natalia L Komarova
Sep 17, 2013·Applied and Environmental Microbiology·Stefan ZdraljevicC Gustavo Pesce
Jan 30, 2016·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Zhenhua HuElaine A Elion
Feb 9, 2012·FEBS Letters·Benjamin A BlountTom Ellis
Sep 1, 2009·Trends in Genetics : TIG·Joanna Masel, Mark L Siegal
Mar 7, 2013·Molecular Microbiology·Kentaro Furukawa, Stefan Hohmann
Feb 18, 2010·Yeast·David PincusOrna Resnekov
Oct 16, 2015·The Journal of Cell Biology·Benjamin WoodsDaniel J Lew
Mar 19, 2014·Current Biology : CB·Chun-Chen KuoDaniel J Lew
Jul 17, 2015·Molecular Biology of the Cell·Michal J NagiecHenrik G Dohlman
Jan 21, 2012·Biochemical Society Transactions·Christopher J Caunt, Craig A McArdle
Jun 30, 2012·Cellular Signalling·Catherine Qiurong PanBoon Chuan Low
Dec 21, 2013·FEMS Microbiology Reviews·Sophie G Martin, Robert A Arkowitz
Dec 9, 2014·Cell Cycle·Tianhai Tian, Angus Harding
Feb 11, 2014·Chemistry & Biology·Ruth Nussinov, Chung-Jung Tsai
Nov 12, 2013·Current Biology : CB·Jacob Stewart-OrnsteinHana El-Samad
Sep 29, 2011·Current Biology : CB·Samyabrata Bhaduri, Peter M Pryciak
Apr 13, 2011·Current Biology : CB·Lee Bardwell
Oct 30, 2008·Current Biology : CB·Lee Bardwell
Jun 22, 2013·Current Biology : CB·F Donelson Smith, John D Scott
Aug 23, 2011·Molecular Cell·Andreas DoncicJan M Skotheim
Nov 13, 2012·Structure·John D LaughlinPhilip V LoGrasso
May 21, 2013·Molecular Cell·Andreas Doncic, Jan M Skotheim
Mar 26, 2009·Chemistry & Biology·Peter M Pryciak
Feb 28, 2013·Biophysical Journal·Alan Bush, Alejandro Colman-Lerner
Jan 15, 2015·Science Signaling·Justin G EnglishHenrik G Dohlman
Nov 12, 2014·Molecular and Cellular Biology·Andrew PitoniakPaul J Cullen
Apr 8, 2016·Molecular Biology of the Cell·Hui KangDaniel J Lew
Jul 28, 2016·Cell Systems·Oguzhan AtayJan M Skotheim
May 5, 2016·Yeast·Damien Coudreuse

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.