Membrane lysis by gramicidin S visualized in red blood cells and giant vesicles

Biochimica Et Biophysica Acta
S SemrauMark Overhand

Abstract

The cationic amphiphilic antimicrobial peptide gramicidin S (GS) is an effective antibiotic. Its applicability is however restricted to topical infections due to its hemolytic activity. In this study, the process of GS induced hemolysis was investigated in detail for the first time. The morphological changes of red blood cells (RBCs) inflicted by GS were visualized and explained in terms of a physical model. The observed fast rupture events were further investigated with giant unilamellar vesicles (GUVs) as model systems for RBCs. Measurements of membrane fluctuations in GUVs revealed that the membrane surface tension was increased after incubation with GS. These findings are in agreement with the hypothesis that amphiphilic peptides induce membrane rupture by an increase in membrane tension.

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Citations

Mar 15, 2016·Biochimica Et Biophysica Acta·A WestJ N Sachs
Oct 23, 2012·Journal of Peptide Science : an Official Publication of the European Peptide Society·Ellen V HacklSergey V Gatash
Jul 23, 2013·Biophysical Journal·Sterling A WheatenPaulo F Almeida
Jan 7, 2015·Saudi Journal of Biological Sciences·Ghulam Md AshrafNaheed Banu
Jul 6, 2017·The Journal of Antibiotics·Kyriacos C Nicolaou, Stephan Rigol
Jan 25, 2020·Beilstein Journal of Organic Chemistry·Sergii AfoninAnne S Ulrich
Nov 12, 2020·ACS Infectious Diseases·Dana Logviniuk, Micha Fridman

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