Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission

Nature Cell Biology
Stefanie SowinskiDaniel M Davis

Abstract

Transmission of HIV-1 via intercellular connections has been estimated as 100-1000 times more efficient than a cell-free process, perhaps in part explaining persistent viral spread in the presence of neutralizing antibodies. Such effective intercellular transfer of HIV-1 could occur through virological synapses or target-cell filopodia connected to infected cells. Here we report that membrane nanotubes, formed when T cells make contact and subsequently part, provide a new route for HIV-1 transmission. Membrane nanotubes are known to connect various cell types, including neuronal and immune cells, and allow calcium-mediated signals to spread between connected myeloid cells. However, T-cell nanotubes are distinct from open-ended membranous tethers between other cell types, as a dynamic junction persists within T-cell nanotubes or at their contact with cell bodies. We also report that an extracellular matrix scaffold allows T-cell nanotubes to adopt variably shaped contours. HIV-1 transfers to uninfected T cells through nanotubes in a receptor-dependent manner. These data lead us to propose that HIV-1 can spread using nanotubular connections formed by short-term intercellular unions in which T cells specialize.

References

Jan 1, 1992·Cell Motility and the Cytoskeleton·T J Mitchison
Apr 1, 1993·Journal of Virology·D S DimitrovM A Martin
Jul 2, 2002·Nature Medicine·Warner C Greene, B Matija Peterlin
May 6, 2003·Science·David McDonaldThomas J Hope
Jan 22, 2004·The Journal of Experimental Medicine·Clare JollyQuentin J Sattentau
Feb 14, 2004·Science·Amin RustomHans-Hermann Gerdes
Feb 27, 2004·The Journal of Biological Chemistry·Fangtian HuangAlexander Sorkin
Jul 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Björn OnfeltDaniel M Davis
Oct 7, 2004·The Journal of Experimental Medicine·Mark J MillerMichael D Cahalan
Jun 14, 2005·Proceedings of the National Academy of Sciences of the United States of America·Herman W FavoreelHans J Nauwynck
Jul 20, 2005·The Journal of Cell Biology·Maik J LehmannWalther Mothes
Sep 20, 2005·Immunity·Simon C Watkins, Russell D Salter
Dec 13, 2005·Current Opinion in Cell Biology·Jan Faix, Klemens Rottner
Nov 3, 2006·Journal of Virology·Marion SourisseauOlivier Schwartz
Dec 23, 2006·Science·Christopher D C AllenJason G Cyster
Jan 12, 2007·The EMBO Journal·Nathalie Sol-FoulonOlivier Schwartz
Feb 13, 2007·Nature Cell Biology·Nathan M ShererWalther Mothes
Apr 17, 2007·FEBS Letters·Hans-Hermann GerdesJoão F V Barroso
May 11, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·D R Daniels, M S Turner

❮ Previous
Next ❯

Citations

May 29, 2010·Archivum Immunologiae Et Therapiae Experimentalis·Kathryn BrownWilson Wong
Nov 7, 2012·Die Naturwissenschaften·Sandra Bloemendal, Ulrich Kück
Apr 4, 2008·Histochemistry and Cell Biology·Steffen GurkeHans-Hermann Gerdes
Aug 15, 2009·Cell and Tissue Research·Werner W FrankeSebastian Pieperhoff
Dec 12, 2012·Cell and Tissue Research·Shunsuke KimuraHiroshi Ohno
Mar 5, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Kate L JonesJohnson Mak
Jul 27, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Irena Kadiu, Howard E Gendelman
Aug 7, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lu-Min ChenYu-Jia Chang
May 1, 2013·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Julijana KristlPetra Kocbek
Aug 6, 2013·The Journal of Infection·Peter-Christian KlöhnJohn Collinge
Oct 15, 2013·Trends in Cell Biology·Raphaël GaudinPhilippe Benaroch
Jun 10, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Jonathan W ArmondMatthew S Turner
Nov 30, 2010·Cell Death and Differentiation·Y WangY Zhang
Dec 12, 2012·Cell Death & Disease·X SunY Zhang
Jul 23, 2013·Cell Death & Disease·N RainyY Kloog
Nov 14, 2012·Immunology and Cell Biology·Yashar Seyed-RazaviHolly R Chinnery
Aug 20, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Candice WillmonRichard Vile
Aug 3, 2012·Nature·Thomas T MurookaThorsten R Mempel
Mar 4, 2009·Nature Cell Biology·Hans-Hermann Gerdes
Dec 2, 2009·Nature Cell Biology·Yuting Zhao, Wei Guo
Feb 10, 2009·Nature Cell Biology·Karine GoussetChiara Zurzolo
Dec 29, 2012·Nature Communications·Xaver SewaldWalther Mothes
Aug 21, 2009·Nature Immunology·Dominika Rudnicka, Olivier Schwartz
Jan 9, 2010·Nature Medicine·Kathryn S Jones, Patrick L Green
May 13, 2008·Nature Reviews. Immunology·Florian Hladik, M Juliana McElrath
Apr 24, 2008·Nature Reviews. Molecular Cell Biology·Daniel M Davis, Stefanie Sowinski
Oct 17, 2008·Nature Reviews. Microbiology·Quentin Sattentau
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Won Gu LeeAli Khademhosseini
May 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·Céline Van den BroekeHerman W Favoreel
Mar 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Anne ChauveauDaniel M Davis
Sep 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Xiang WangHans-Hermann Gerdes
Nov 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Nancy Van ProoyenGenoveffa Franchini
Nov 6, 2009·AIDS Research and Human Retroviruses·Robert SealyJulia L Hurwitz
Feb 23, 2011·AIDS Research and Human Retroviruses·Weihong LaiChin-Ho Chen
Aug 25, 2011·Journal of Experimental Botany·Rosemary G White, Deborah A Barton
Sep 20, 2008·Current Opinion in HIV and AIDS·Li Wu
Dec 17, 2009·AIDS·Deborah J AndersonKenneth H Mayer
Nov 19, 2010·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Ines Frank, Melissa Robbiani

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.