Membrane of Candida albicans as a target of berberine

BMC Complementary and Alternative Medicine
Nataša ZorićJosipa Vlainić

Abstract

We investigated the mechanisms of anti-Candida action of isoquinoline alkaloid berberine, active constituent of medically important plants of Barberry species. The effects on membrane, morphological transition, synthesis of ergosterol and the consequent changes in membrane permeability have been studied. Polarization and lipid peroxidation level of the membrane following berberine treatment have been addressed. Minimal inhibitory concentration (MIC) of berberine against C. albicans was 17.75 μg/mL. Cytotoxic effect of berberine was concentration dependent, and in sub-MIC concentrations inhibit morphological transition of C. albicans cells to its filamentous form. Results showed that berberine affects synthesis of membrane ergosterol dose-dependently and induces increased membrane permeability causing loss of intracellular material to the outer space (DNA/protein leakage). Berberine also caused membrane depolarization and lipid peroxidation of membrane constituents indicating its direct effect on the membrane. Moreover, ROS levels were also increased following berberine treatment indicating further the possibility of membrane damage. Based on the obtained results it seems that berberine achieves its anti-Candida activity by affe...Continue Reading

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Citations

Nov 2, 2018·World Journal of Microbiology & Biotechnology·Jaegoo KimKi-Young Kim
Jan 26, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sourav DasTamás Kőszegi
Jul 31, 2020·Medicinal Research Reviews·Xiao-Fei ShangKuo-Hsiung Lee
Jul 28, 2019·Antimicrobial Resistance and Infection Control·Bahman KhamenehBibi Sedigheh Fazly Bazzaz
May 16, 2020·Frontiers in Microbiology·Xiujuan ZhangXiaoyuan Wang
Jun 20, 2020·Biotechnology Letters·Xiaoxue HuangYan Li
Feb 23, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chen Wen XiaoGuo Lian Bao
Feb 25, 2019·Lasers in Medical Science·Chiaki Tsutsumi-AraiKazuhito Satomura
Apr 3, 2020·ACS Infectious Diseases·Laura N SilvaAndré L S Santos
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Josipa VlainićTomislav Šmuc

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Methods Mentioned

BETA
MDA

Software Mentioned

GraphPad Prism

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