Membrane order conservation in raft and non-raft regions of hepatocyte plasma membranes from thermally acclimated rainbow trout

Biochimica Et Biophysica Acta
John K Zehmer, J R Hazel

Abstract

Homeoviscous adaptation (HVA), the thermal conservation of membrane fluidity/order at different body temperatures, has been observed to varying degrees in different membranes. However, HVA has not been studied in raft and non-raft regions of the plasma membrane (PM) separately. Rafts are ordered PM microdomains implicated in signal transduction, membrane traffic and cholesterol homeostasis. Using infrared spectroscopy, we measured order in raft-enriched PM (raft) and raft-depleted PM (RDPM) isolated from hepatocytes of rainbow trout (Oncorhynchus mykiss) acclimated to 5 and 20 degrees C. We found approximately 130% and 90% order compensation in raft and RDPM, respectively, suggesting their independent regulation. Raft was more ordered than RDPM in the warm-acclimated trout, a difference fully explained by a 58% enrichment of cholesterol, compared to RPDM. Unexpectedly, raft and RDPM from cold-acclimated trout did not differ in cholesterol content or order. Freezing the membrane samples during preparation had no effect on order. Treatment with cyclodextrin depleted cholesterol by 36%, 56%, and 55%, producing significant decreases in order in raft and RDPM from warm-acclimated trout and RDPM from cold-acclimated trout, respective...Continue Reading

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Citations

Dec 12, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Graham BrogdenDieter Steinhagen
Jul 11, 2016·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Teresa M FonovichJorge Herkovits
Apr 9, 2008·Physiological Reviews·Rafael LindenRicardo R Brentani
Aug 30, 2019·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Elie FarhatJean-Michel Weber
Dec 20, 2013·The Journal of Experimental Biology·Scott A L HaywardAndrew R Cossins

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