Membrane permeability characteristics of metaphase II mouse oocytes at various temperatures in the presence of Me2SO

Cryobiology
Y AgcaJ K Critser

Abstract

In this study, the hydraulic conductivity (Lp), Me2SO permeability (PMe2SO), and the reflection coefficients (sigma) and their activation energies were determined for Metaphase II (MII) mouse oocytes by exposing them to 1.5 M Me2SO at temperatures of 30, 20, 10, 3, 0, and -3 degrees C. These data were then used to calculate the intracellular concentration of Me2SO at given temperatures. Individual oocytes were immobilized using a holding pipette in 5 microliters of an isosmotic PBS solution and perfused with precooled or prewarmed 1.5 M Me2SO solutions. Oocyte images were video recorded. The cell volume changes were calculated from the measurement of the diameter of the oocytes, assuming a spherical shape. The initial volume of the oocytes in the isoosmotic solution was considered 100%, and relative changes in the volume of the oocytes after exposure to the Me2SO were plotted against time. Mean (means +/- SEM) Lp values in the presence of Me2SO were (LpMe2SO) at 30, 20, 10, 3, 0 and -3 degrees C were determined to be 1.07 +/- 0.03, 0.40 +/- 0.02, 0.18 +/- 0.01, 7.60 x 10(-2) +/- 0.60 x 10(-2), 5.29 x 10(-2) +/- 0.40 x 10(-2), and 3.69 x 10(-2) +/- 0.30 x 10(-2) microns/min/atm, respectively. The PMe2SO values were 3.69 x 10(-3)...Continue Reading

References

May 1, 1990·The Journal of Membrane Biology·M TonerD R Armant
Jul 1, 1990·Molecular Reproduction and Development·C VincentS J Quick
Jun 1, 1989·Journal of in Vitro Fertilization and Embryo Transfer : IVF·M H Johnson
Oct 1, 1989·Cryobiology·C J ToupinL E McGann
Jun 1, 1980·The Journal of Experimental Zoology·S JackowskiP Mazur
Jan 1, 1996·Human Reproduction·A I YounisJ D Biggers
Feb 1, 1958·Biochimica Et Biophysica Acta·O KEDEM, A KATCHALSKY

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Citations

Dec 21, 2000·ILAR Journal·Y Agca
Jun 13, 2015·Reproductive Biology and Endocrinology : RB&E·Joana E MatosRosa M L N Pereira
Jul 24, 2001·Biology of Reproduction·L KeskintepeJ K Critser
May 11, 2005·The Journal of Reproduction and Development·Prudencio B PedroMagosaburo Kasai
Nov 11, 2009·Bulletin of Experimental Biology and Medicine·M A PogorelovaV A Golichenkov
Jan 1, 2018·Reproduction, Fertility, and Development·Gary D Smith, Shuichi Takayama
Dec 7, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Willem F WolkersHarald Sieme

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