Membrane permeability to water measured by microfluidic trapping of giant vesicles

Soft Matter
Tripta BhatiaRumiana Dimova

Abstract

We use a microfluidic method to estimate the water permeability coefficient (p) of membranes. As model lipid membranes we employ giant unilamellar vesicles (GUVs) composed of palmitoyloleoyl phosphatidylcholine and cholesterol (10 mol%). We have developed a microfluidic device with multiple chambers to trap GUVs and allow controlled osmotic exchange. Each chamber has a ring-shaped pressure-controlled valve which upon closure allows isolation of the GUVs in a defined volume. Opening the valves leads to a rapid fluid exchange between the trapping region and the microchannel network outside, thus allowing precise control over solution concentration around the GUVs contrary to other experimental approaches for permeability measurements reported in the literature. The area and volume changes of individual vesicles are monitored with confocal microscopy. The solute concentration in the immediate vicinity of the GUVs, and thus the concentration gradient across the membrane, is independently assessed. The data are well fitted by a simple model for water permeability which assumes that the rate of change in volume of a GUV per unit area is linearly proportional to concentration difference with permeability as the proportionality constan...Continue Reading

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Citations

Jul 6, 2021·Biochimica Et Biophysica Acta. Biomembranes·Yunuen Avalos-PadillaRumiana Dimova
Jul 4, 2021·Science Advances·Stine Lauritzen SønderJesper Nylandsted

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Methods Mentioned

BETA
optical microscopy
biopsy
chips
chip
confocal microscopy
phase contrast microscopy

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