Membrane permeabilization by non-steroidal anti-inflammatory drugs

Biochemical and Biophysical Research Communications
W TomisatoTohru Mizushima

Abstract

The cytotoxicity of non-steroidal anti-inflammatory drugs (NSAIDs) is involved in the formation of NSAID-induced gastric lesions. The mechanism(s) behind these cytotoxic effects, however, is not well understood. We found here that several NSAIDs tested caused hemolysis when employed at concentrations similar to those that result in cytotoxicity. Moreover, these same NSAIDs were found to directly permeabilize the membranes of calcein-loaded liposomes. Given the similarity in NSAID concentrations for cytotoxic and membrane permeabilization effects, the cytotoxic action of these NSAIDs may be mediated through the permeabilization of biological membranes. Increase in the intracellular Ca(2+) level can lead to cell death. We here found that all of NSAIDs tested increased the intracellular Ca(2+) level at concentrations similar to those that result in cytotoxicity. Based on these results, we consider a possibility that membrane permeabilization by NSAIDs induces cell death through increase in the intracellular Ca(2+) level.

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Citations

Sep 1, 2005·Digestive Diseases and Sciences·Yasuhiro AraiTohru Mizushima
Sep 28, 2005·Digestive Diseases and Sciences·Wataru TomisatoTohru Mizushima
May 15, 2009·The Journal of Biological Chemistry·Shintaro SuemasuTohru Mizushima
Feb 4, 2009·Antioxidants & Redox Signaling·Masanori Kitamura
Mar 2, 2010·Biological & Pharmaceutical Bulletin·Naoki YamakawaTohru Mizushima
Feb 2, 2008·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Tohru Mizushima
Oct 17, 2007·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Tohru Mizushima
Jul 13, 2012·The Journal of Veterinary Medical Science·Lee-Shuan LinNobuo Sasaki
Aug 16, 2012·Drug Metabolism and Pharmacokinetics·Naoki YamakawaTohru Mizushima
Oct 2, 2009·Journal of Clinical Biochemistry and Nutrition·Kazuhide HiguchiEiji Umegaki
Apr 12, 2013·Mediators of Inflammation·Mau SinhaTej P Singh
May 15, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Takashi KatsuTohru Mizushima
Dec 17, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Takashi KatsuSatoshi Nakao
Dec 27, 2011·Colloids and Surfaces. B, Biointerfaces·Raquel SilvaArtur Cavaco-Paulo
Mar 25, 2008·Neuropharmacology·Martin GamerdingerChristian Behl
Mar 21, 2007·Biochemical and Biophysical Research Communications·Tomoaki IshiharaTohru Mizushima
Oct 7, 2015·Journal of Natural Medicines·Seyyed Ali MardIraj Ahmadi
Feb 6, 2010·Biochemical Pharmacology·Tomoaki IshiharaTohru Mizushima
Dec 5, 2006·Disease-a-month : DM·Gina Gill Glass
Oct 2, 2012·Biochemical Pharmacology·Shintaro SuemasuTohru Mizushima
Apr 16, 2009·Biochimica Et Biophysica Acta·Marcela Manrique-MorenoKlaus Brandenburg
Oct 28, 2016·Gut and Liver·Jaeyong HanTong Wang
Mar 22, 2014·Bioorganic & Medicinal Chemistry·Naoki YamakawaTohru Mizushima
May 25, 2010·Pharmaceuticals·Tohru Mizushima
Jun 18, 2016·The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi·Young Kwang Shim, Nayoung Kim
Sep 2, 2006·The Journal of Biological Chemistry·Mayuko AburayaTohru Mizushima
Oct 13, 2006·The Journal of Pharmacy and Pharmacology·Majella E Lane, Mi-Jeong Kim
Sep 19, 2008·American Journal of Physiology. Renal Physiology·Maro OkamuraMasanori Kitamura

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