Membrane phase state and the rearrangement of hematopoietic cell surface receptors.

Molecular and Cellular Biology
P WilliamsonR A Schlegel

Abstract

Transformed murine hematopoietic cells of several lineages bound the fluorescent membrane probe merocyanine 540, whereas their normal counterparts did not. Similar selective binding was reproduced in artificial liposomes which bound this probe above their phase transition temperature, but not below it. The regions of the membrane to which merocyanine 540 binds along with the receptors for the lectin concanavalin A, but not the receptors for the lectin wheat germ agglutinin, were rearranged during the course of induced differentiation of erythroleukemia cells. Based on these findings, we propose a model of hematopoietic cell surface differentiation in which proteins such as concanavalin A receptors, which are destined for removal from the plasma membrane, are specifically associated with disordered, liquid-like lipid domains which can be visualized with merocyanine 540. For the specific case of erythroid differentiation, these domains and their associated proteins are collected at the region of the membrane where nuclear extrusion occurs and are eliminated from the reticulocyte plasma membrane by the enucleation event.

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·C R KahnJ S Flier
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J SchlessingerI Pastan
Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J SchlessingerE L Elson
Jun 5, 1973·Biochemistry·E J Shimshick, H M McConnell
Jan 1, 1974·The Journal of Membrane Biology·L B CohenC H Wang
Dec 5, 1958·Annals of the New York Academy of Sciences·G A FISCHER

❮ Previous
Next ❯

Citations

Aug 1, 1986·British Journal of Haematology·H R ChoeM P Westerman
Jan 1, 1989·Journal of Cellular Physiology·B J Del BuonoR A Schlegel
Jul 1, 1984·Experimental Cell Research·G P CoferR A Schlegel
Mar 1, 1982·Journal of Cellular Physiology·B M PhelpsR A Schlegel
Mar 1, 1986·Journal of Cellular Physiology·B J Del BuonoR A Schlegel
Jan 1, 1993·Journal of Hematotherapy·F Sieber
Jun 13, 2003·Biology of Reproduction·Eiman M-E Aboagla, T Terada
Jan 1, 1991·Photochemistry and Photobiology·J B Feix, B Kalyanaraman
Dec 1, 1987·Photochemistry and Photobiology·F Sieber
Dec 1, 1987·Photochemistry and Photobiology·D P ValenzenoM Milne
Jun 9, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·E AlarcónJ C Scaiano
Jun 1, 1991·Journal of Photochemistry and Photobiology. B, Biology·M HoebekeA van de Vorst
Mar 23, 1984·Biochimica Et Biophysica Acta·D J FreidusP Williamson
Nov 6, 1986·Biochimica Et Biophysica Acta·A ArduiniA Stern
Feb 14, 1987·Biochimica Et Biophysica Acta·V GuarcelloV Rizza
Aug 30, 1985·Biochimica Et Biophysica Acta·R A SchlegelP Williamson
Jul 27, 1983·Biochimica Et Biophysica Acta·P WilliamsonR A Schlegel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.