Membrane potential, anion and cation conductances in Ehrlich ascites tumor cells.

The Journal of Membrane Biology
I H LambertF Jørgensen

Abstract

The fluorescence intensity of the dye 1,1'-dipropylox-adicarbocyanine (DiOC3-(5] has been measured in suspensions of Ehrlich ascites tumor cells in an attempt to monitor their membrane potential (Vm) under different ionic conditions, after treatment with cation ionophores and after hypotonic cell swelling. Calibration is performed with gramicidin in Na+-free K-/choline-media, i.e., standard medium in which NaCl is replaced by KCl and cholineCl and where the sum of potassium and choline is kept constant at 155 mM. Calibration by the valinomycin "null point" procedure described by Laris et al. (Laris, P.C., Pershadsingh, A., Johnstone, R.M., 1976, Biochim, Biophys. Acta 436:475-488) is shown to be valid only in the presence of the Cl- -channel blocker indacrinone (MK196). Distribution of the lipophilic anion SCN- as an indirect estimation of the membrane potential is found not to be applicable for the fast changes in Vm reported in this paper. Incubation with DiOC3-(5) for 5 min is demonstrated to reduce the Cl permeability by 26 +/- 5% and the NO3- permeability by 15 +/- 2%, while no significant effect of the probe could be demonstrated on the K+ permeability. Values for Vm, corrected for the inhibitory effect of the dye on the ...Continue Reading

References

Feb 6, 1978·The Journal of Membrane Biology·U V LassenB Vestergaard-Bogind
Jul 14, 1977·Biochimica Et Biophysica Acta·G Burchkhardt
Jan 1, 1976·Annual Review of Biochemistry·B C Pressman
Jun 30, 1976·The Journal of Membrane Biology·A Waggoner
Jun 17, 1976·Biochimica Et Biophysica Acta·P C LarisR M Johnstone
Aug 1, 1990·The Journal of Membrane Biology·H Völkl, F Lang
Jan 1, 1985·Pflügers Archiv : European journal of physiology·A Di StefanoR Greger
Jan 1, 1985·The Journal of Membrane Biology·B Vestergaard-BogindP Christophersen
Jan 1, 1986·The Journal of Membrane Biology·E K HoffmannL O Simonsen
Jan 1, 1986·The Journal of Membrane Biology·K Eskesen, H H Ussing
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R L Hudson, S G Schultz
Apr 1, 1989·Physiological Reviews·E K Hoffmann, L O Simonsen
Jun 1, 1986·Acta Physiologica Scandinavica·J E Dürr, E H Larsen
Sep 1, 1986·The American Journal of Physiology·B KramhøftF Jørgensen
May 1, 1988·Pflügers Archiv : European journal of physiology·H Völkl, F Lang
May 1, 1987·Pflügers Archiv : European journal of physiology·E GstreinF Lang
Aug 21, 1986·Biochimica Et Biophysica Acta·W D Dawson, T C Smith
Feb 12, 1987·Biochimica Et Biophysica Acta·W D Dawson, T C Smith
Apr 1, 1985·Journal of Cellular Physiology·O KempskiA Baethmann
Feb 26, 1974·Biochimica Et Biophysica Acta·C De Cespedes, H N Christensen
Jan 1, 1980·Biochimica Et Biophysica Acta·T J RinkR Y Tsien
Dec 30, 1980·The Journal of Membrane Biology·P Läuger
Mar 8, 1982·Biochimica Et Biophysica Acta·M ValdeolmillosB Herreros
Aug 1, 1982·Journal of Cellular Physiology·T C Smith
Jan 1, 1984·The Journal of Membrane Biology·J W Bowen, C Levinson
Jul 25, 1984·Biochimica Et Biophysica Acta·B SarkadiA Rothstein
May 1, 1983·The Journal of Physiology·E K Hoffmann, I H Lambert
Aug 1, 1982·Journal of Cellular Physiology·R M JohnstoneA A Eddy
Dec 1, 1982·The Journal of General Physiology·S GrinsteinA Rothstein
Jul 31, 1980·Biochemical and Biophysical Research Communications·T C Smith, S C Robinson
Apr 28, 1955·The Journal of Physiology·A L HODGKIN, R D KEYNES

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Citations

Dec 11, 1997·The Journal of Experimental Zoology·E K Hoffmann
May 1, 1989·The Journal of Membrane Biology·I H Lambert
Jan 1, 1993·The Journal of Membrane Biology·I H Lambert, E K Hoffmann
Mar 27, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·E K Hoffmann, C Hougaard
Sep 30, 2010·Acta Physiologica·E K Hoffmann, S F Pedersen
Jun 15, 2010·Journal of Cancer Research and Clinical Oncology·Benjamin KaterAram Prokop
Dec 20, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Else Kay Hoffmann
Jan 19, 2007·American Journal of Physiology. Cell Physiology·Else K Hoffmann, Stine F Pedersen
Apr 18, 2008·American Journal of Physiology. Cell Physiology·Martin Barfred FriisIan Henry Lambert
Jan 8, 2009·Physiological Reviews·Else K HoffmannStine F Pedersen
May 8, 2009·American Journal of Physiology. Cell Physiology·Ian Henry LambertElse Kay Hoffmann
Jul 16, 2010·American Journal of Physiology. Cell Physiology·Signe Skyum KirkegaardElse Kay Hoffmann
Oct 29, 2010·Chemistry : a European Journal·Liliane A OnambeleAram Prokop
Feb 11, 1998·Physiological Reviews·F LangD Häussinger
Apr 4, 2014·American Journal of Physiology. Cell Physiology·Ian Henry LambertPer Amstrup Pedersen
Feb 10, 2021·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Sina M HopffAram Prokop
Apr 26, 1996·Biochimica Et Biophysica Acta·R J Thomas-Young, C Levinson
Jan 16, 1999·Biochimica Et Biophysica Acta·S PedersenE K Hoffmann
Feb 23, 1994·Biochimica Et Biophysica Acta·A Ballarin-DentiH Kuroda
Dec 12, 1991·Biochimica Et Biophysica Acta·B Sarkadi, J C Parker

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