Membrane potential shapes regulation of dopamine transporter trafficking at the plasma membrane

Nature Communications
Ben D RichardsonHabibeh Khoshbouei

Abstract

The dopaminergic system is essential for cognitive processes, including reward, attention and motor control. In addition to DA release and availability of synaptic DA receptors, timing and magnitude of DA neurotransmission depend on extracellular DA-level regulation by the dopamine transporter (DAT), the membrane expression and trafficking of which are highly dynamic. Data presented here from real-time TIRF (TIRFM) and confocal microscopy coupled with surface biotinylation and electrophysiology suggest that changes in the membrane potential alone, a universal yet dynamic cellular property, rapidly alter trafficking of DAT to and from the surface membrane. Broadly, these findings suggest that cell-surface DAT levels are sensitive to membrane potential changes, which can rapidly drive DAT internalization from and insertion into the cell membrane, thus having an impact on the capacity for DAT to regulate extracellular DA levels.

References

Jan 1, 1996·Neuropharmacology·M JaberM G Caron
Nov 5, 1997·Advances in Pharmacology·L J DeFelice, A Galli
Sep 26, 1997·Movement Disorders : Official Journal of the Movement Disorder Society·M JaberM G Caron
Aug 26, 1998·Drug and Alcohol Dependence·S G Amara, M S Sonders
Mar 29, 2000·The Journal of Biological Chemistry·M L MundorfR M Wightman
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·C SaundersA Galli
Aug 30, 2001·Proceedings of the National Academy of Sciences of the United States of America·H HastrupJ A Javitch
Feb 1, 2002·Biochemical and Biophysical Research Communications·Lynette C DawsAurelio Galli
Feb 28, 2002·Behavioural Brain Research·Bertha K MadrasAlan J Fischman
Jul 20, 2002·Progress in Neurobiology·André Nieoullon
Sep 28, 2002·Nature Neuroscience·Susan L IngramSusan G Amara
Jan 31, 2003·The Journal of Biological Chemistry·Habibeh KhoshboueiAurelio Galli
Feb 8, 2003·Brain Research. Molecular Brain Research·James D FosterRoxanne A Vaughan
May 15, 2003·The Journal of Biological Chemistry·Tatiana SorkinaAlexander Sorkin
Sep 17, 2003·The Journal of Pharmacology and Experimental Therapeutics·Limen Chi, Maarten E A Reith
Nov 13, 2003·European Journal of Pharmacology·Kristopher M Kahlig, Aurelio Galli
Nov 13, 2003·European Journal of Pharmacology·Ole V Mortensen, Susan G Amara
Oct 7, 2004·Neuropharmacology·Nancy R Zahniser, Alexander Sorkin
Feb 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kristopher M KahligAurelio Galli
Aug 20, 2005·The Journal of Biological Chemistry·Manuel MirandaAlexander Sorkin
May 11, 2006·Molecular Pharmacology·Kristopher M KahligAurelio Galli
Apr 25, 2007·The American Journal on Addictions·Peter W Kalivas
Jun 30, 2007·Annual Review of Neuroscience·Wolfram Schultz
Dec 3, 2008·The Journal of Biological Chemistry·J Shawn GoodwinHabibeh Khoshbouei
Jan 27, 2009·Nature Methods·Fedor V SubachVladislav V Verkhusha
Feb 6, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tatiana SorkinaAlexander Sorkin
Mar 13, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Cheryse A FurmanMargaret Gnegy
May 29, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jacob EriksenUlrik Gether
Jun 30, 2009·Seminars in Cell & Developmental Biology·Nancy R Zahniser, Alexander Sorkin
Aug 22, 2009·Nature Reviews. Molecular Cell Biology·Barth D Grant, Julie G Donaldson
Aug 28, 2009·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·George F Koob, Nora D Volkow
Mar 6, 2010·Annals of the New York Academy of Sciences·Kyle C Schmitt, Maarten E A Reith
Jun 17, 2010·The Journal of Biological Chemistry·Jacob EriksenUlrik Gether
Aug 24, 2010·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Daniel J Lodge, Anthony A Grace
Nov 5, 2010·Arquivos de neuro-psiquiatria·Quirino CordeiroHomero Vallada

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Citations

Mar 3, 2019·The Journal of Biological Chemistry·Joseph J LebowitzHabibeh Khoshbouei
May 30, 2019·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Joshua D GrossVincent Setola
Dec 5, 2019·Movement Disorders : Official Journal of the Movement Disorder Society·Mariana H G MonjeCarmen Cavada
May 2, 2020·Journal of Neurochemistry·Suzanne O NolanErin S Calipari
Jun 11, 2020·Molecular Biology of the Cell·Brian B SilverCeleste M Nelson
Dec 22, 2019·Scientific Reports·Guglielmo SchianoOlivier Devuyst
Dec 8, 2019·Biophysical Journal·Md Mizanur Rahman MoghalMasahito Yamazaki
Jan 21, 2021·American Journal of Physiology. Cell Physiology·Minkyoung KimKyungpyo Park
Aug 7, 2021·RSC Medicinal Chemistry·Gisela Andrea Camacho-HernandezAmy Hauck Newman
Nov 9, 2021·Journal of Neuroscience Research·Rosaria FormisanoHarbinder S Dhillon

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Methods Mentioned

BETA
fluorescence microscopy
confocal microscopy
pharmacotherapy
transfection
protein assay

Software Mentioned

ImageJ
Pro
Igor
Excel
Image Studio
GraphPad
NIS Elements
SPSS
Prism5

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