PMID: 6109002Oct 1, 1980Paper

Membrane potentials in cell-free preparations from guinea pig cerebral cortex: effect of depolarizing agents and cyclic nucleotides

Journal of Neurochemistry
C R CrevelingJ W Daly

Abstract

The distribution of [3H]triphenylmethylphosphonium ion between the medium and vesicular entities was examined in a cell-free, particulate preparation from guinea pig cerebral cortex. This distribution followed the Nernst relationship with regard to the external potassium ion concentration and, in physiological media, indicated the maintenance of a mean trans-membrane potential ranging from -58 to -78 mV. The neurotoxins batrachotoxin, veratridine, and grayanotoxin I, partially depolarized the preparation. Tetrodotoxin blocked the depolarization by batrachotoxin, veratridine, and gray-anotoxin I. The depolarization by these neurotoxins was potentiated by the presence of anemone toxin II and presumably reflected the response of vesicular components of neuronal origin. An additional potassium-sensitive depolarization probably represented the response of vesicular components of glial origin with an apparent transmembrane potential of -8 to -35 mV. No correlation could be demonstrated between changes in transmembrane potential and stimulation of cyclic AMP generation by a variety of agents in this preparation.

References

Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E F GrollmanL D Kohn
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D LichtshteinA J Blume
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D LichtshteinA J Blume
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S RamosL D Kohn
Dec 30, 1975·Annals of the New York Academy of Sciences·E HeinzC Pietrzyk
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·G Romey Lazdunski
Jan 1, 1972·International Review of Neurobiology·M H Evans
Oct 1, 1974·The Journal of Physiology·T Narahashi, I Seyama
Apr 1, 1974·Analytical Biochemistry·Y SalomonM Rodbell
Jan 1, 1969·Ergebnisse der Physiologie, biologischen Chemie und experimentellen Pharmakologie·W Ulbricht
May 1, 1964·The Journal of General Physiology·T NARAHASHIW R SCOTT

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Citations

Mar 1, 1981·Cellular and Molecular Neurobiology·G B BrownE X Albuquerque
Apr 15, 1994·Pharmacy World & Science : PWS·M J HigginsT W Stone
May 1, 1990·Brain Research. Developmental Brain Research·M L FiszmanJ L Barker
Jan 1, 1989·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·M Knipper, H Breer
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·F GusovskyJ W Daly
Feb 1, 1993·Journal of Neurochemistry·L BettendorffE Schoffeniels
Jun 1, 1982·Journal of Neurochemistry·A PastuszkoM Erecińska
Jan 1, 1983·Journal of Neurochemistry·S BerlD D Clarke
Oct 25, 2007·Progress in Brain Research·Sokratis TrellakisGoetz Lehnerdt
Jul 6, 2014·Neurotoxicology·Pablo ValdiviaTimothy J Shafer
Aug 1, 1986·Journal of Neurochemistry·F MayorF Mayor

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