Membrane preconcentration-capillary electrophoresis-mass spectrometry in the analysis of biologically derived metabolites and biopolymers

Biomedical Chromatography : BMC
S Naylor, A J Tomlinson

Abstract

On-line capillary electrophoresis-mass spectrometry (CE-MS) is finding increased use in the analysis of a wide variety of chemically diverse complex mixtures. It is characterized by minimal sample loss and enhanced separation efficiencies as compared to conventional techniques such as high-performance liquid chromatography-mass spectrometry (HPLC-MS). However, the major limitation of both CE and CE-MS is the limited sample loading capacity of conventional CE capillaries. Typical loading volumes are approximately 1-100 nL, which afford optimal CE performance, and this is in stark contrast to the 1-100 microL commonly injected onto capillary HPLC columns. The limited loading of CE leads to relatively poor concentration limits of detection. In this work a unique method for analyte preconcentration with CE is described. A cartridge containing an impregnated membrane is installed at the inlet of the CE capillary, and we term this approach membrane preconcentration-CE (mPC-CE), and in conjunction with mass spectrometry, mPC-CE-MS. It allows both on-line sample concentration and cleanup and concentration limits of detection of fg/mL are possible using this approach. The analysis of in vivo derived metabolites, peptides, and proteins i...Continue Reading

Citations

Sep 26, 2000·Biomedical Chromatography : BMC·O Y Al-Dirbashi, K Nakashima
Dec 31, 1998·Electrophoresis·S J KokU A Brinkman
Aug 13, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·F TagliaroM Marigo
Dec 9, 1998·Drug Metabolism Reviews·E UsukiN Castagnoli
Dec 7, 2007·Electrophoresis·F W Alexander TempelsGerhardus J de Jong
Aug 28, 2001·Journal of Chromatography. a·M ValcárcelA Ríos
Nov 20, 2001·Chemical Reviews·K B Tomer

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