Membrane Processing and Steady-State Regulation of the Alternative Peroxisomal Import Receptor Pex9p

Frontiers in Cell and Developmental Biology
Markus RudowitzWolfgang Schliebs

Abstract

Import of peroxisomal matrix proteins with a type 1 peroxisomal targeting signal (PTS1) in Saccharomyces cerevisiae is facilitated by cytosolic import receptors Pex5p and Pex9p. While Pex5p has a broad specificity for all PTS1 proteins independent of the growth conditions, Pex9p is only expressed in fatty-acid containing media to mediate peroxisomal import of the two malate synthases, Mls1p and Mls2p, as well as the glutathione transferase Gto1p. Pex5p-cargo complexes dock at the peroxisomal membrane, translocate their cargo-protein via a transient pore and are recycled into the cytosol for a further round of import. The processing of Pex5p has been shown to require a complex network of interactions with other membrane-bound peroxins, as well as decoration with ubiquitin as signal for its ATP-dependent release and recycling. Here, we show that the alternative receptor Pex9p requires the same set of interacting peroxins to mediate peroxisomal import of Mls1p. However, while Pex5p is rather stable, Pex9p is rapidly degraded during its normal life cycle. The steady-state regulation of Pex9p, combining oleate-induced expression with high turnover rates resembles that of Pex18p, one of the two co-receptors of the PTS2-dependent targ...Continue Reading

References

May 1, 1989·The Journal of Cell Biology·S J GouldS Subramani
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M P Yaffe, G Schatz
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·I Van der LeijH F Tabak
Oct 9, 2001·The Journal of Biological Chemistry·P E Purdue, P B Lazarow
Oct 10, 2006·Biochimica Et Biophysica Acta·Wolfgang Schliebs, Wolf-H Kunau
Jan 9, 2007·The Journal of Biological Chemistry·Sébastien Léon, Suresh Subramani
Apr 25, 2007·The Journal of Cell Biology·Harald W PlattaRalf Erdmann
Jun 7, 2007·The Journal of Biological Chemistry·Chris WilliamsBen Distel
Feb 12, 2009·The Journal of Biological Chemistry·Cláudia P GrouJorge E Azevedo
Feb 16, 2010·Nature Cell Biology·Michael MeineckeRalf Erdmann
Jun 15, 2011·The Journal of Biological Chemistry·Mykhaylo O DebelyyRalf Erdmann
Oct 25, 2011·The Journal of Biological Chemistry·Astrid HenselRalf Erdmann
Nov 8, 2011·The Journal of Biological Chemistry·Markus KunzeJohannes Berger
Dec 4, 2014·Cell Reports·Romain ChristianoTobias C Walther
Aug 16, 2015·The Journal of Biological Chemistry·Daniel EffelsbergRalf Erdmann
Dec 18, 2015·Cell Reports·Malayko Montilla-MartinezRalf Erdmann
Nov 3, 2016·Journal of Cell Science·Daniel EffelsbergRalf Erdmann

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Methods Mentioned

BETA
ubiquitination
Fluorescence
fluorescence microscopy

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