Membrane progestin receptors alpha and gamma in renal epithelium

Biochimica Et Biophysica Acta
Julie LemaleGilles Crambert

Abstract

Sex hormones have broader effects than regulating reproductive functions. Recent identification of membrane progestin receptors expressed in kidney prompted us to investigate their putative involvement in the renal effects of this hormone. We first focused our investigations on mPRalpha and gamma by analyzing three parameters 1/ their distribution along the mouse nephron and their subcellular location in native kidney, 2/ the ability of progesterone to stimulate ERK pathway and/or Ca(2+) release from internal stores in native kidney structures and 3/ the cellular localization of mPRalpha and its molecular determinants in heterologous expression system. We observed that 1/ mPRalpha expression is restricted to proximal tubules of both male and female mice whereas mPRgamma exhibits a much broader expression all along the nephron except the glomerulus, 2/ mPRalpha and gamma are not localized at the plasma membrane in native kidney, 3/ this expression does not permit either progesterone-induced ERK phosphorylation or Ca(2+) release and 4/ in HEK transfected cells, mPRalpha localizes in the endoplasmic reticulum (ER) due to a C-terminal ER retention motif (-KXX). Therefore, we have characterized mPRs in kidney but their role in renal...Continue Reading

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Citations

Feb 27, 2013·Pflügers Archiv : European journal of physiology·Amel SalhiGilles Crambert
Sep 20, 2011·The Journal of Biological Chemistry·Oforiwa A GorlekuLuke H Chamberlain
Oct 22, 2008·Human Reproduction Update·B GellersenJ J Brosens
Apr 30, 2016·Journal of the American Society of Nephrology : JASN·Thomas SeppiJudith Lechner
Jul 6, 2013·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J B GraceliM R Moysés
Feb 7, 2015·American Journal of Physiology. Renal Physiology·Carol M Herak-KrambergerIvan Sabolić
May 1, 2010·Biomolecular Concepts·Amel SalhiGilles Crambert

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