Membrane properties of D-erythro-N-acyl sphingomyelins and their corresponding dihydro species

Biophysical Journal
M KuikkaJ P Slotte

Abstract

We have prepared acyl chain-defined D-erythro-sphingomyelins and D-erythro-dihydrosphingomyelins and compared their properties in monolayer and bilayer membranes. Surface pressure/molecular area isotherms of D-erythro-N-16:0-sphingomyelin (16:0-SM) and D-erythro-N-16:0-dihydrosphingomyelin (16:0-DHSM) show very similar packing properties, except that the expanded-to-condensed phase transition (crystallization) occurs at a lower surface pressure for 16:0-DHSM. The measured surface potential was generally about 100 mV less for 16:0-DHSM monolayers compared to 16:0-SM monolayers. The condensed domains (crystals) that formed in 16:0-SM monolayers as a function of compression displayed star-shaped morphology when viewed under an epifluorescence microscope. 16:0-DHSM monolayers did not form similar crystals upon compression. 16:0-DHSM was degraded much faster by sphingomyelinase from Staphylococcus aureus than 16:0-SM (10-fold difference in enzyme activity needed for comparable hydrolytic rate). Cholesterol desorption from 16:0-DHSM to cyclodextrin was slightly slower (approximately 20%) than the rate measured from 16:0-SM monolayers (at 60 mol % cholesterol). The bilayer melting temperature of 16:0-DHSM was 47.7 degrees C (DeltaH 8....Continue Reading

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Citations

Nov 13, 2009·European Biophysics Journal : EBJ·Bodil WesterlundJ Peter Slotte
Mar 5, 2004·Chemistry and Physics of Lipids·M Cecilia Yappert, Douglas Borchman
Aug 7, 2002·Current Opinion in Structural Biology·Erwin London
Feb 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Britta BrüggerHans-Georg Kräusslich
Aug 9, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Avraham AshkenaziYechiel Shai
Apr 22, 2010·Journal of Lipid Research·Douglas Borchman, Marta C Yappert
Mar 21, 2008·Journal of Pharmaceutical Sciences·Daryl C DrummondDmitri B Kirpotin
Jan 21, 2016·Biophysical Journal·Tomokazu YasudaJ Peter Slotte
Apr 24, 2013·Current Opinion in Cell Biology·Christian KloseKai Simons
Mar 5, 2016·Pharmacological Reports : PR·Kamil BieniasHalina Car
May 18, 2013·Experimental Eye Research·Jihong TongThomas J McIntosh
Mar 16, 2016·Biochimica Et Biophysica Acta·Sara García-LinaresJ Peter Slotte
Nov 9, 2004·Chemistry and Physics of Lipids·Richard M Epand, Raquel F Epand
Mar 24, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Juan Palacios-OrtegaJ Peter Slotte
Nov 29, 2005·Chemistry and Physics of Lipids·Christine Grauby-Heywang, Jean-Marie Turlet
Aug 10, 2005·Biochimica Et Biophysica Acta·Bohdana TérováJ Peter Slotte
Jul 28, 2010·Chemistry & Biology·Catarina R VieiraFélix M Goñi
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Sep 26, 2003·Biophysical Journal·Rodrigo F M de AlmeidaManuel Prieto
Dec 4, 2003·Biophysical Journal·S W ChiuH Larry Scott
Nov 17, 2009·Biochimica Et Biophysica Acta·Anders BjörkbomJ Peter Slotte
Sep 3, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Kevin LetchfordHelen Burt
Feb 27, 2016·AIDS·Huihui ChongYuxian He
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Apr 7, 2017·Nutrition Reviews·Gregory H Norris, Christopher N Blesso

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